摘要
利用SSR分子标记结合"拟测交"策略,以荷包红鲤抗寒品系和柏氏鲤远缘杂交所产生F1代为亲本及其F2代为作图群体,应用Windows Map Manager2.0软件的标记回归法进行乳酸脱氢酶(Lactate dehydrogenase,LDH)活性数量性状基因座单标记定位分析。在乳酸脱氢酶性状的标记回归研究中,共发现12个标记与LDH性状关联,对性状的贡献率为4.00%～10.00%,其中HLJE222达到极显著水平(P<0.01)。为了进一步验证,利用已有的生物信息学工具,将与性状连锁的EST序列与公共数据库中的核酸序列进行同源搜索分析,发现标记HLJE222的EST序列与斑马鱼DAZ associated protein1的mRNA序列相匹配(相似性为94%),与公共数据库中的已知蛋白序列进行相似性比较,同样发现HLJE222的EST与斑马鱼DAZ associated protein1相匹配(相似性为97%)。结果表明HLJE222位点与影响乳酸脱氢酶活性相关的基因连锁。
Microsatellite markers were used to make marker regression analysis on activity of lactate dehydrogenase based on double pseudo-testcross strategy using Windows Map Manager2.0 software. The parents that came from the cross between progenies of Hebao-cold tolerance red carp and Barbless carp and F2 progenies were used as segregating populations. For maker regression, a total of 12 markers associated with activity of lactate dehydrogenase were significant at P〈0.05 and HLJE222 was significant at P〈0.01. The variance explained by these loci, ranged from 4.00% to 10.00%. Lo- cus HLJE222 was closely linked to the gene related to activity of lactate dehydrogenase of common carp. For further identification, EST-SSR markers were used to screen the protein and nucleotide database using bioinformatics tools in order to find the homologies. High sequence similarities of HLJE222 marker were observed with the nucleotide sequence of DAZ associated protein lmRNA of zebrafish（94%）, and protein sequence of DAZ associated protein 1（97%）. DAZ protein is one of the short chain dehydrogenases, which is an important enzyme in the process of glucose metabolism in the organisms. This family contains a wide variety of dehydrogenases. This indicates that locus HLIE222 was closely linked to the gene associated with activity of lactate dehydrogenase of common carp.
出处
《遗传》
CAS
CSCD
北大核心
2009年第4期407-411,共5页
Hereditas（Beijing)
基金
国家重点基础研究发展规划(973计划)项目(编号:2004CB117405)资助
关键词
鲤鱼
标记回归分析
乳酸脱氢酶
拟测交策略
EST—SSR
common carp（Cyprinus carpio）
marker regression
lactate dehydrogenase
double pseudo-testcross strategy
EST-SSR
