摘要
中国根霉１２（Ｒ．ｃｈｉｎｅｓｉｓ１２）菌株产生的抗生物质存在于发酵液中，首先经过饱和度为７０％硫酸铵盐析处理，再经半透膜透析去离子后，通过Ｓｅｐｈａｄｅｘ－Ｇ７５凝胶柱层析，浓缩含有活性组分的层析洗脱液，得到抗生物质粗制品。经ＨＰＬＣ和薄板层析法证明为一纯组分。
The antibiotic substance produced from Rhizopus chinesis 12 exist in fermentation solution.The solution was treated by salting-out(70% saturated ammonium sulfate), then deionized by dialysis,filtrated by sephadex-G75 gel,condensed the active substance.At last the antibiotic substance preparation was obtained.The analysis result of HPLC and TLC proved the antibiotic preparation is pure substance.The molecular weight of the antibiotic substance is 48000 Dalton,tested by sephadex-G75 gel column chromatography.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
1998年第2期32-36,共5页
Food and Fermentation Industries
关键词
中国根霉
抗生物质
分离
提纯
分子量
发酵工业
Rhizopus chinesis,antibiotic substance,isolation and purification,molecular wehight.
