摘要
目的探讨乳果糖和庆大霉素灌胃对梗阻性黄疸(梗黄)大鼠应激性溃疡的影响及可能机制。方法雄性Wistar大鼠40只随机分4组:A组,结扎胆总管并以乳果糖和庆大霉素灌胃;B组,结扎胆总管并以葡萄糖灌胃;C组,假手术并用乳果糖和庆大霉素灌胃;D组,假手术并用葡萄糖灌胃。先行手术,14 d后再将4组大鼠分别行灌胃7 d,然后以水浸束缚应激诱导建立应激性溃疡模型,并分别检测门静脉血内毒素值和胃黏膜溃疡指数(UI,Guth评分)。结果A组门静脉血内毒素值及UI显著下降,B组门静脉血内毒素值及UI升高;C,D组门静脉血内毒素值及UI均处于较低水平。结论内毒素血症是加剧梗黄状态下应激性溃疡发生的重要原因之一。乳果糖和庆大霉素灌胃可降低血内毒素水平,有利于降低梗黄大鼠的总体应激性溃疡的发生率。
Objective To investigate the effect and the mechanism of intragastric administration of gentamicin and lactulose on stress ulcer in rats with obstructive jaundice. Methods Forty male Wistar rats were divided into 4 groups ： Jaundice （ Ligation of the bile duct ） and intragastric administration of gentamicin and lactulose （group A ） , jaundice with intragastric glucose （group B ） , sham operation with intragastris gentamicin and lactulose （ group C ） ; and sham operation with intragastric glucose （ group D ）. Two weeks after the operation, the respective intragastric administration was given for 7 days, and then the water immersion and restraint stress induction procedures were performed in the 4 groups. Endotoxemia levels in portal vein were measured by the Limulus test and the ulcer index was calculated. Results Group A showed significantly lower ulcer index and lower level of endotoxemia compared to group B, while the levels of endotoxemia and ulcer index in group B were increased, and group C and D also showed lower ulcer index and lower level of endotoxemia. Conclusions Endotoxemia is an important aggravating factor for stress ulcer in obstructive jaundice. Intragastric administration of lactulose and gentamicin can decrease the level of endotoxemia which is beneficial to reduce the incidence of stress ulcer in rats with obstructive jaundice.
出处
《中国普通外科杂志》
CAS
CSCD
北大核心
2009年第3期260-263,共4页
China Journal of General Surgery
关键词
胃溃疡/病因学
应激
黄疸
阻塞性
内毒素
Stomach Ulcer/etiol
Stress
Jaundice, Obstructive
Endotoxemia
