摘要
目的:建立同时测定蓝芩口服液中盐酸小檗碱和黄芩苷的含量方法。方法:采用高效液相色谱法,色谱柱:Zorbax XDB-C18柱(4.6×150mm,5μm),流动相:甲醇-0.5%磷酸溶液梯度洗脱,比例为0～16.5min,甲醇38%～40%,流速1ml/min;16.8～18min,甲醇99%,流速1.6ml/min;18.1～22min,甲醇38%,流速1.6ml/min;22.1～23min,甲醇38%,流速1ml/min。检测波长为275nm,柱温30℃。结果:盐酸小檗碱在6.8～65.28μg/ml,黄芩苷在31.2～299.52μg/ml范围内与峰面积呈良好的线性关系。加样回收率在97.0%～101.3%,RSD小于2.0%。结论:本法操作简便、结果准确、分离效果好,可用于蓝芩口服液中盐酸小檗碱和黄芩苷含量的测定。
Objective ：To simultaneously determine the contents of berberine hydroehloride and baiealin in Lanqin Oral Liquid via an HPLC method. Method：The HPLC with a C18 column （4.6×150 mm ,5 μm） was used. The mobile phases were as follows： the methanol- 0.5% orthophosphoric acid （0-16.5 min,methanol 38%-40% ,V=1 ml/min;16.8~18 min,methanol 99% ,V-1.6ml/min;18.1-22min, methanol 38%, V= 1.6ml/min ; 22.1-23 min, methanol 38%, V= 1 ml/min.）.The wavelength for detection was located at 275nm.Resuh ：The linear ranges of berberine hydrochloride and baicallin were 6.8-65.28 μg/ml and 31.2-299.52 μg/ml, respectively, both showing a good linar relationship, and the average spotting recoveries were higher than 97.0%. The coefficient of variation was smaller than 2.0%.Conclusion：The method is convenient and can be used for controlling the quality of Lanqin Oral Liquid.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2009年第2期210-211,共2页
Journal of Chongqing Medical University
