摘要
目的:研究中药白术(Atractylodes macrocephala)对正常晚孕子宫平滑肌细胞及经白细胞介素6(Interleukin-6,IL-6)作用后的子宫平滑肌细胞钙依赖钾通道电流(BKca)的影响,以探讨白术对早产时宫缩的抑制作用及电生理机制。方法:①急性分离人晚孕子宫平滑肌细胞,采用Axopatchl-D膜片钳放大器测全细胞模式下的单个平滑肌细胞大电导的钙离子依赖钾通道电流(BKca);②测定灌流液中含不同浓度的白术时,子宫平滑肌细胞BKca的变化;③测定灌流液中含IL-6(10ng/ml)时全细胞模式下BKca;继而测定灌流液中含有不同浓度的白术时,子宫平滑肌细胞BKca的变化。结果:10ng/mlIL-6可抑制BKca的幅值约(36.9±13.7)%(n=10,P<0.01),灌流液中含2mg/ml白术时增强BKca的幅值约(36.7±22.6)%(n=10,P<0.01),对于IL-6作用后的细胞,2mg.mL-1白术增强BKca的幅值约(45.2±13.7)%(n=10,P<0.01)。结论:白术可以兴奋晚孕子宫平滑肌细胞及IL-6作用后的子宫平滑肌细胞的BKca,且对IL-6作用后的子宫平滑肌细胞BKca的兴奋作用强于对正常子宫平滑肌细胞,证明它有助于维持妊娠时子宫平滑肌的膜电位和静息状态,为开发中药治疗早产提供一定的理论依据。
Objective： To explore the repression effect of atractylodes macroeephala on the Ca^2＋ - activated K^＋ currents of human pregnant myometrium smooth muscle cells with or without interleukin - 6, discuss the electrophysiology mechanism of atractylodes macroceph- ala. Methods： Cells were separated acutely from human pregnant myometrium smooth muscle, Ca^2＋ - activated K ＋ currents were detected by an Axopatchl- D amplifier under conventional whole cell patterns. The process was divided into three groups ： group A （ atraetylodes mac- rocephala was added into perfusate）, group B （ interleukin - 6 was added into perfusate）, group C （ atractylodes macroeephala was added in- to perfusate after interleukin -6 had been added in） . Results： When added in 10 ng/ml interleukin -6, the increase degree of BKca was （36.9%±13.7% ） compared with control group （P 〈 0. 01 ）. 2 mg/ml atractylodes macrocephala respectively enforced BKca by （36.7% ±22. 6% ） or （45.2% ± 13.7% ） with or without IL - 6 （ P 〈 0. 01 ） . Conclusion： Atractylodes macroeephala can enhance the BKca of human pregnant myometrium smooth muscle cells with or without Interleukin -6. The augmented effection of atraetylodes macrocephala on the BKca with interleukin - 6 is more powerful than that without it. Atraetylodes macrocephala can help to maintain the membrance potentials and smooth muscle quiescence in the pregnant human myometrium.
出处
《中国妇幼保健》
CAS
北大核心
2009年第3期366-368,共3页
Maternal and Child Health Care of China
关键词
白术
白细胞介素6
钙离子依赖的钾通道
子宫平滑肌
Atractylodes macrocephala
Interleukin-6
Ca^2＋- activated K^＋ currents
Myometrium smooth muscle
