摘要
为选择一种适合法夫酵母发酵虾青素的工业碳源,分别以麦芽糖浆、果葡糖浆、标准糖浆3种淀粉糖为碳源做发酵试验。试验结果表明,以麦芽糖浆、果葡糖浆和标准糖浆为碳源摇瓶培养法夫酵母,虾青素的体积产率及细胞产率间没有显著差异;分别以3种淀粉糖为碳源,在20L机械搅拌罐中补料分批培养法夫酵母的发酵规律相似,但以标准糖浆为碳源的虾青素细胞产率和虾青素得率高于其它2种淀粉糖,分别达到0.38mg虾青素/g干酵母和0.25mg虾青素/g葡萄糖;以标准糖浆为碳源,在75L机械搅拌罐中补料分批培养法夫酵母的发酵规律与20L罐的发酵规律一致,但虾青素的细胞产率为0.33mg/g干菌体,略低于20L罐的细胞产率;虾青素的得率为0.34mg/L,略高于20L罐的虾青素得率。
Cultivation of Phaffia rhodozyma by using three starch syrups as carbon sources were studied for selection an-industrial carbon source for astaxanthin production. When the yeast was cultured in shake-flask, no great differences were detected in the astaxanthin yield in terms of volume and biomass with the use of maltose syrup, fructose syrup, and standard syrup, respectively. When the yeasts were cultured by fed batch in 20 L fermenter, it was suggested that the common rules of the termentation are very similar, but the astaxanthin yield in terms of sugar and astaxatnhtin yield in terms of biomass is higer than other two syrups, which are 0.25 mg/g sugar and 0.38 mg/g dry cell weight. It indicated that standard syrup is the best for culture Phaffia rhodozyma for astaxanthin production. The results of fed batch fermentation in 75 L fermenter using standard syrup as carbon source showed that the general rule were consistent with the results in 20 L fermenter. But higer astaxanthin yield were harvested. After 65 h fermentation, the astaxanthin yield in terms of biomass was kept stably at 0.35 mg/g, and the yield of astaxanthin in terms of sugar could reach to 0.34 mg/g.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2009年第1期29-34,共6页
Journal of Chinese Institute Of Food Science and Technology
基金
福建省科技计划重点项目(2006H0033)
福建省青年人才创新基金项目(2005Z035)
关键词
法夫酵母
虾青素
淀粉糖
碳源
工业发酵
Phaffta rhodozyma Astaxanthin Starch syrup Carbon source Industrial fermentation
