摘要
用7个我国当前流行的条锈菌生理小种对V9128-3的抗条锈性进行了评价,表明本易位系对我国优势流行小种具有良好的抗病性。以Su-4对V9128-3与铭贤169配置的F1、BC1F1、F2及F3代群体进行了遗传分析,并对其中1个F2群体进行了SSR标记,再用BC1F1群体的部分单株和F3家系进行连锁标记的初步验证。遗传分析表明了V9128-3对Su-4的抗病性由1对显性核基因独立控制,从219对SSR引物中筛选到2个位于2AL上的该基因YrHV(暂命名)两侧的标记Xgwm356和Xwmc658,遗传距离分别为8.5和5.6cM,所用部分BC1F1单株和F3家系验证了该2个标记与YrHV连锁性。将此标记可用于小麦抗条锈病分子标记辅助育种。
The Triticum aestivum-Haynaldia villosa translocation line V9128-3 was inoculated with seven dominant races of Puccinia striiforms f. sp. tritici prevalent in China, the results of resistance evaluation showed that the translocation line V9128-3 possessed resistance to the current epidemic yellow rust races. V9128-3, susceptible cultivar Mingxian 169 and their progeny of F1 ,F2, BC1F1 ,F3 were inoculated with race Su-4 ,one of the F2 progeny were used for developing molecular markers of stripe rust resistence gene in V9128-3 ,a portion of BC1F1 individuals and F3 lines were used to validate the the linked markers. The results of inheritance analysis showed that V9128-3 resistance to Su-4 was controlled by one dominant gene. Two markers Xgwm356 and Xwmc658 located on 2AL from 219 SSR primer combinations were found linked to YrHV（ temporarily designated）. YrHV was 8.5 cM from Xgwm356and 5.6 cM from Xwmc658, respectively, the two sites linked to YrHV were validated by a portion of BC1F1 individuals and F3 lines. These markers would be used for breeding new wheat cultivars with marker-assisted selection.
出处
《植物病理学报》
CAS
CSCD
北大核心
2009年第1期67-75,共9页
Acta Phytopathologica Sinica
基金
教育部长江学者和创新团队发展计划资助项目(200558)
关键词
簇毛麦
易位系
条锈病
遗传分析
SSR标记
Haynaldia villosa
translocation lines
stripe rust
genetic analysis
SSR molecular mapping
