摘要
从牛脑垂体中提取总ＲＮＡ，分离ｍＲＮＡ，反转录获得ｃＤＮＡ，ＰＣＲ扩增获得长为３８０ｂｐ的牛促卵泡激素α亚基ｃＤＮＡ片段，将它克隆于ｐＵＣ１９中，进行序列分析，结果表明：所克隆的α亚基基因编码区序列与Ｅｒｗｉｎ所发表的序列基本相同，仅编码第２４位赖氨酸的密码有差异，同时将所获基因的核苷酸序列及相应氨基酸序列与人、啮齿类的同类基因相比较，具有很高的同源性．
Total RNA was prepared from pituitary of bovine.mRNA was isolated from the total RNA. cDNA was synthesized as template used in PCR for amplification of cDNA for α subunit of bovine follicle stimulating hormone. Sequence analysis showed that the obtained cDNA fragment is 380 bp and all these nucleotides except one coding for Lys of No24 are as same as that Erwin reported. Comparison of bovine, human and rodent indicated that the amino acid and nucleotide sequences of the α subunit in these mammalian species are highly conservative.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1998年第2期159-162,共4页
Progress In Biochemistry and Biophysics
基金
国家自然科学基金
关键词
牛促卵泡激素
促卵泡激素
Α亚基
CDNA
序列分析
α subunit of bovine follicle stimulating hormone,cDNA, nucleotide sequence analysis
