摘要
目的检测CD4+CD25+CD127low/-T细胞在强直性脊柱炎(ankylosing spondylitis,AS)患者外周血的表达,并分析其与CD4+CD25+FOXP3+T细胞的相关性。方法以21例AS患者外周血为研究组,20例类风湿关节炎(rheumatoid arthritis,RA)患者和24名正常人外周血作为对照组,采用三色直接荧光素标记法和多参数流式细胞仪检测外周血CD4+CD25+CD127low/-T细胞、CD4+CD25highT细胞及CD4+CD25+FOXP3+T细胞的比例,同时检测外周血C反应蛋白(C-reactive protein,CRP)、血沉(ESR)、免疫球蛋白、补体等水平以及HLA-B27并进行相关性分析。结果与正常对照组相比,AS患者外周血CD4+CD25+CD127low/-及CD4+CD25+FOXP3+T细胞百分率降低(均P<0.05),CD4+CD25highT细胞百分率升高(P<0.05);CD4+CD25+CD127low/-T细胞与CD4+CD25+FOXP3+T细胞呈正相关(r=0.589,P=0.021),与CRP、ESR、血小板及免疫球蛋白(IgG、IgA、IgM)和补体(C3、C4)、BASDAI、HLA-B27等均无明显相关性(均P>0.05)。AS患者外周血CD4+CD25+CD127low/-T细胞、CD4+CD25+FOXP3+T细胞和CD4+CD25highT细胞的百分率与RA患者外周血比较差异均无统计学意义(P>0.05)。结论AS患者外周血CD4+CD25+CD127low/-T细胞降低,且与CD4+CD25+FOXP3+T细胞呈正相关,提示在调节性T细胞研究中CD127具有替代FOXP3的可能性。
To detect the expression of CD4^＋CD25^＋CD127^low/-T cells in the peripheral blood of patients with ankylosing spondylitis （AS） and analyse its correlation with and CD4^＋CD25^＋FOXP3^＋T cells. Methods Twenty-one AS patients, 20 rheumatoid arthritis （RA） patients and 24 healthy controls were enrolled in this study, CD4CD4^＋CD25^＋CD127^low/-T CD4^CD25^high, and CD4^＋CD25^＋FOXP3^＋T cells of their peripheral blood were analyzed by 3-color flow cytometry. Levels of ESR, CRP, immunoglobulin and complement, HLA-B27 were also measured. Results In comparison to healthy controls, percents of CD4^＋CD25^＋CD127^low/-Tcells and CD4^＋CD25^＋FOXP3^＋T cells in the peripheral blood of AS patients were decreased （P〈 0.05 in each case）, and the percent of CD4^＋CD25^high T cells was increased （P〈 0.05）; CD4^＋CD25^＋CD127^low/-T cells were positively correlated with CD4^＋CD25^＋FOXP3^＋T cells （r = 0.589, P =0.021）, but not with the levels of BASDAI. CRP, each case）. Percents of CD4^＋CD25^＋CD127^low/-T cells, ESR and complements and HLA-B27 （P〉 0.05 in CD4^＋CD25^＋FOXP3^＋T cells and CD4^＋CD25^highT cells in the peripheral blood of AS patients showed no significant difference with that of RA patients （P 〉 0.05）. Conclusion The percent of CD4CD4^＋CD25^＋CD127^low/-Tcells is decreased in the peripheral blood of AS patients and positively correlates with CD4^＋CD25^＋FOXP3^＋T cells. CD127 may be a surrogate marker of FOXP3 in studying regulatory T cells.
出处
《中华临床免疫和变态反应杂志》
2008年第4期266-271,共6页
Chinese Journal of Allergy & Clinical Immunology
基金
安徽省国际科技合作项目(07080703022)
安徽省临床医学重点学科应用技术项目(05A008)
