期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

应用合成的SARS病毒S蛋白重叠肽筛选B细胞表位 被引量：6

Identification of Antibody Epitopes in the Severe Acute Respiratory Syndrome Coronavirus Spike Glycoprotein by Peptide Scanning
原文传递
导出
摘要 目的筛选SARS病毒S蛋白的B细胞表位。方法使用SARS-CoV S DNA疫苗免疫BALB/c小鼠,获得SARS病毒S蛋白的免疫血清。人工合成包含169条部分氨基酸序列重叠的SARS-CoV S蛋白的多肽库。将多肽片段包被ELISA板,利用免疫小鼠血清,通过抗体结合试验来筛选SARS病毒S蛋白的线性B细胞表位。并将筛选结果与使用B细胞表位分析软件预测的结果进行比较。结果使用重叠肽合成法筛选到SARS-CoV蛋白两条多肽片段S335-352和S442-458,能与免疫动物血清特异性结合,与使用Bcipep数据库预测B细胞表位的结果相一致。结论鉴定了两个新的SARS病毒S蛋白B细胞表位。 Objective Identification of antibody epitopes in the severe acute respiratory syndrome eoronavirus spike glycoprotein. Method BALB/c mice were immunized with SARS-CoV S DNA vaccine, and sera were collected from immunized mice. 169 peptides contained overlapped peptide pool which covered the whole SARS-CoV S protein were synthesized. ELISA plate was coated with the peptides and B cell epitopes were determined by antibody binding test by sera from DNA vaccine immunized mice. The results were compared with that predicted by Bcipep data. Result Sera from SARS-CoV S DNA vaccine immunized mice reacted with the peptides of S335-352 and S442-458 among 169 SARS- CoV S peptides. These two antibody epitopes also can be predicted by Bcipep data. Conclusion Two new B cell epitopes of SARS-CoV spike protein were identified in mice following SARS-CoV S DNA vaccination.
作者 黄俊 吴长有
机构地区 中山大学基础医学院免疫学教研室 广州医学院基础医学院病原学教研室
出处 《热带医学杂志》 CAS 2009年第2期161-163,184,共4页 Journal of Tropical Medicine
基金 国家科技部863项目(No.2007AA02Z415) 广州市2008年第5批科学技术经费项目(No.2008J1-C141-3)
关键词 SARS—CoV S蛋白 B细胞表位 重叠肽合成法 SARS-CoV Spike protein B cell epitope peptide scanning
分类号 R392 [医药卫生—免疫学]
  • 相关文献

参考文献13

  • 1Peiris JSM, Yuen KY, Osterhaus ADME, et al. The severe acute respiratory syndrome [J]. N Engl J Med, 2003,349 ( 25 ) : 2431-2441.
  • 2Heike H, Kim H, Andrea M, et al. S protein of severe acute respiratory syndrome-a.ssociated eoronavirus mediates entry into hepatoma cell lines and is targeted by neutralizing antibodies in infected patients [J]. ] Virol, 2004,78(12):6134-6142.
  • 3Nie Y, Wang G, Shi X, et al. Neutralizing antibodies in patients with severe acute respiratory syndrome-associated coronavirus infection [J]. J Infect Dis, 2004, 190(6) : 1119- 1126.
  • 4Wong VW, Dai D, Wu AK, et al. Treatment of severe acute respiratory syndrome with convalescent plasma [J]. Hong Kong Med J, 2003,9(3) : 199-201
  • 5Yang ZY, Kong WP, Huang Y, et al. A DNA vaccine induces SARS coronavirus neutralization and protective immunity in mice [J]. Nature, 2004,428(6982) :561-564.
  • 6Kanta S, Josephine M, Leatrice V, et al. Prior infection and passive transfer of neutralization antibody prevent replication of severe acute respiratory syndrome eoronovirus in the respiratory tract of mice [J]. Virol, 2004,78(7):3572-3577.
  • 7Saha S, Bhasin M, Raghava GP. Bcipep: a database of B- cell epitopes [ J ]. BMC Genomics, 2005,6 ( 1 ) : 79.
  • 8Zhang H, Wang G, Li J, et al. Identification of an antigenic determinant on the S2 domain of the severe acute respiratory syndrome coronavirus spike glycoprotein capable of inducing neutralizing antibodies [J]. J Virol, 2004,78 (13) :6938-6945.
  • 9He Y, Li J, Du L, et al. Identification and characterization of novel neutralizing epitopes in the receptor-binding domain of SARS-CoV spike protein: revealing the critical antigenic determinants in inactivated SARS-CoV vaccine [J ]. Vaccine, 2006,24 ( 26 ) : 5498- 5508.
  • 10He Y, Zhou Y, Wu H, et al. Identification of immunodominant sites on the spike protein of severe acute respiratory syndrome (SARS) coronavirus : implication for developing SARS diagnostics and vaccines [J]. J Immunol, 2004,173(6) :4050-4057.

二级参考文献19

  • 1凌世淦 宋晓国 胡巍.SARS病毒多表位免疫原的研究[J].解放军医学杂志,2003,28:6-8.
  • 2Ksiazek TG,Erdman D,Goldsmith CS,et al.SARS Working Group.A novel coronavirus associated with severe acute respiratory syndrome[J].N Engl J Med,2003,348(20):1953.
  • 3Pratelli A,Tinelli A,Decaro N,et al.Safety and efficacy of a modified-live canine coronavirus vaccine in dogs[J].Vet Microbiol,2004,99 (1):43.
  • 4Rota PA,Oberste MS,Monroe SS,et al.Characterization of a novel coronavirus associated with severe acute respiratory syndrome[J].Science, 2003,300(5624):1394.
  • 5Gallagher TM,Buchmeier MJ.Coronavirus spike proteins in viral entry and pathogenesis[J].irology,2001,279(2):371.
  • 6Li W,Moore MJ,Vasilieva N,et al.Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus[J].Nature,2003,426(69 65):450.
  • 7Wong SK,Li W,Moore MJ,et al.A 193-amino acid fragment of the SARS coronavirus Sprotein efficiently binds angiotensin-converting enzyme 2[J].J Biol Chem,2004,279(5):3197.
  • 8Marco A Marra, Steven JM Jones, Caroline R Astell, et al. The genome sequence of the SARS-associated coronavirus. Science, 2003, 300(5624): 1394-1399.
  • 9Drosten C,Gunther S,Preiser W,et al.Identification of a novel coronavirus in patients with severe acute respiratory syndrome [J].N Engl J Med,2003,348 (20):1967-1976.
  • 10Rota PA,Oberste MS,Monroe SS,et al.Characterization of a novel coronavirus associated with severe acute respiratory syndrome [J].Science,2003,300 (5 624):1394-1399.

共引文献4

同被引文献65

引证文献6

二级引证文献6

热带医学杂志
2009年 第2期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部