摘要
目的探讨胶质瘤细胞血小板源生长因子Ｂ链的纯合二聚体（ＰＤＧＦＢＢ）及其受体（ＰＤＧＦＲ）基因表达和ＰＤＧＦＲ活化水平在胶质瘤发生、发展中的作用。方法用原位杂交和免疫组化染色观察了７３例不同级别的人胶质瘤组织标本。结果６２例（８４９％）的肿瘤细胞表达ＰＤＧＦＢｍＲＮＡ，其阳性率和阳性肿瘤细胞含量均随肿瘤恶性程度升高而递增。ＰＤＧＦＲα、ＰＤＧＦＲβ和这两种受体及其信号传递通路活化标记物酪氨酸磷酸化蛋白（ＰＴｙｒ）的阳性率均为１００％。这３种阳性肿瘤细胞密度（个／００５ｍｍ２）彼此间均呈正相关（ｒ＝０８３８～０８９７，Ｐ＜００１），并均随肿瘤恶性程度及肿瘤细胞ＰＤＧＦＢｍＲＮＡ表达水平升高而同步增加，差异均有显著性（Ｐ＜００５～００１）。但各肿瘤组内两种受体阳性肿瘤细胞密度间无显著性差异（Ｐ＞００５）。结论胶质瘤细胞普遍存在ＰＤＧＦＢＢ自分泌环，ＰＤＧＦＲα和ＰＤＧＦＲβ在该自分泌环中均起重要作用，该自分泌环活性异常增加可能对ＰＤＧＦＢＢ及其受体表达有正反馈诱导作用，并可能在胶质瘤发生及恶性进展过程中发挥重要作用。
Objective To investigate the effect of PDGFBB autocrine loop on the development and progression of human gliomas. Methods 73 human glioma specimens of different stages were studied using in situ hybridization and immunohistochemical techniques. Results 62 (84 9%) of 73 cases expressed PDGFB mRNA. The positive rate and the amount of PDGFB mRNA increased correspondingly with the degree of malignancy. All of 73 gliomas expressed PDGFR α, PDGFR β and phosphotyrosine protein (P Tyr ). The positive cell densities of PDGFR α, PDGFR β and P Tyr correlated positively with one another and increased with the malignancy as well as the expression level of PDGFB mRNA in the gliomas. However, there was no significant difference between positive cell densities of PDGFR α and PDGFR β in any glioma group with different grading. Conclusions PDGFBB autocrine loop existed generally in the glioma cells, the expression levels of PDGFBB and PDGFRs were well correlated with the tumor grading and the positive cell density of P Tyr which could objectively reflect the activity of PDGFRs and the signal pathways. It′s suggested that abnormally increased activity of PDGFBB autocrine loop might play an important role in the development and progression of gliomas.
出处
《中华病理学杂志》
CAS
CSCD
北大核心
1998年第2期109-112,共4页
Chinese Journal of Pathology
关键词
神经胶质瘤
血小板源
生长因子
受体
二聚体
Glioma
Platelet derived growth factor
Receptors
platelet derived growth factor
