摘要
研究了热水浸提法从人参茎叶中提取水溶性多糖。体内实验测定小鼠免疫器官指数,体外实验测定腹腔巨噬细胞吞噬中性红(3-氨基-6甲氨基-2甲基吩嗪盐酸盐)能力,并采用噻唑蓝比色法(MTT)测定小鼠脾淋巴细胞增殖能力。结果表明,人参茎叶多糖含量为81.67%。体内、外实验显示,人参茎叶多糖能增加小鼠免疫器官的总质量并能明显提高腹腔巨噬细胞吞噬能力及脾细胞增殖能力。
The hot-water-dipping method was used to extract the polysaccharide from ginseng leaves and stems. With in vivo experiment, the mouse immune system indexes were measured. With in vitro experiment, the mouse macrophage function was studied. The proliferation of mouse spleen cell was measured by MTT assay. The results showed that the ginseng leaves and stems contained 81.67% polysaccharides. The polysaccharide increased the mouse immune system index, and also improved the swallow ability of abdominal cavity macrophage and the multiplication ability of spleen cell obviously in mouse.
出处
《中国酿造》
CAS
北大核心
2009年第3期56-58,共3页
China Brewing
基金
辽宁省科技攻关计划(2007211001)
辽宁省教育厅创新团队计划(2007T009)
关键词
人参茎叶多糖
提取
免疫功能
polysaccharide from ginseng leaves and stems
extraction
immunological function
