摘要
目的观察地塞米松对大鼠坐骨神经损伤后脊髓运动神经元形态学的影响。方法27只成年Wis-tar雄性大鼠随机分为4组。神经损伤组:大鼠右侧股外侧切口,钳夹右侧坐骨神经造成神经损伤;地塞米松组:于神经损伤后局部肌肉间隙内注射地塞米松0.5mg/(kg.d);生理盐水组:在神经损伤后注射等量生理盐水;正常对照组:不做任何处理。手术后不同时间段分别取各组动物L4～L6节段脊髓,制做石蜡切片,观察脊髓运动神经元形态及数量的变化。结果神经损伤组可见同侧脊髓前角运动神经元胞体变小,尼氏体数目减少,模糊不清。地塞米松组神经损伤后的脊髓前角运动神经元和尼氏体的形态明显改善,数量增加。结论大鼠坐骨神经损伤后局部应用地塞米松可改善脊髓前角运动神经元的形态结构,恢复蛋白质合成功能。
Objective To study the effect of dexamethasone on the recovery of motor neuron in anterior horn of spinal cord following sciatic nerve injury. Methods Twenty-seven male Wistar rats were randomly divided into four groups. Nerve injury group： the right sciatic nerve was injured by elampingl Dexamethasone group; dexamethasone 0.5 mg/（kg · d） applied to injured sites immediately; Normal saline group： normal saline was injected to injured sites; Control group： without any intervention. The L4--6 spinal cords were removed, stained with HE and the number and morphological changes of Nissl bodies in anterior horn motor neurons observed. Results In nerve injury group, the number of neuron and Nissl bodies decreased, the neurons became smaller, but in dexamethasone group, the number of neuron and Nissl bodies increased, and the neurons showed almost normal morphological appearance. Conclusion The morphological structure of the anterior horn motor neurons of spinal cord following the nerve injury can be improved by local administration of dexamethasone.
出处
《青岛大学医学院学报》
CAS
2009年第1期44-46,共3页
Acta Academiae Medicinae Qingdao Universitatis
基金
山东省科技发展计划项目(2005GG4202014)
