摘要
采用ＡｌＣｌ３诱导，建立Ａｌｚｈｅｉｍｅｒ病（ＡＤ）模型大鼠。用维生素Ｅ（ＶＥ）（５ｍｇ／１００ｇ体重·ｄ－１）对ＡＤ模型大鼠进行灌胃治疗。通过行为测试，光镜形态学观察，用免疫细胞化学ＡＢＣ结合图像定量分析的方法，对ＡＤ大鼠行为改变，海马结构ＣＡ１区淀粉样蛋白免疫反应阳性神经元的形态和数目、胞体平均截面积和光密度以及β淀粉样蛋白的沉积变化进行观察。结果显示ＶＥ组大鼠治疗３个月和５个月后，受电击次数和潜伏期较对照组明显减少和延长（Ｐ＜００１），３个月与５个月之间有显著性差异（Ｐ＜００１）；ＶＥ组大鼠海马结构ＣＡ１区淀粉样蛋白免疫反应阳性神经元的形态和数目、胞体平均截面积和光密度值，３个月和５个月较对照组均有明显减少和降低（Ｐ＜００１），３个月和５个月之间也有显著性差异（Ｐ＜００１），β淀粉样蛋白样反应染色减少或消失。说明ＶＥ可以改善ＡＤ模型大鼠的学习记忆，其作用机制是通过抑制和清除海马结构ＣＡ１区β淀粉样蛋白的沉积来实现的，作用效果随治疗时间的延长而更加明显。本实验研究结果为临床应用ＶＥ治疗老年性痴呆提供了形态学依据。
おhe purpose of our study was to explore the mechanism of VE to treat Alzheimer's disease(AD). AD model rats was treated by VE with 5mg/100g bodyweigh/d,po.The alternation of rats behavior,the number and the average area as well as absorbance(ASO) of APLi neurons and βAP depositing in CA1 region of hippoampal formation of AD model rat's by the methods of behavior test ,light microscope morphological observation as well as immunocytochemical combining image analysis. The results showed that VE could improve the AD model rat's ability of learn and memorizing, which mechanisms were achieved by preventing βAP from depositing and clearing deposited βAP. The studies give a histological support for the usage of VE treating AD in the clinic.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
1998年第2期95-97,共3页
Chinese Journal of Gerontology
基金
国家自然科学基金
