摘要
目的:探讨老龄大鼠脑缺血再灌注后海马神经元超微结构变化,以及线粒体形态改变程度及性质。方法:建立老年大鼠脑缺血动物模型,正常对照组(A组)、缺血30min再灌注6h(B组)、12h(C组)、24h(D组)、48h(E组)和7d组(F组),用透射电镜观察海马神经元的超微结构变化,通过计算机图像分析系统对线粒体形态计量分析。结果:B组海马神经元超微结构与A组相同,E组和F组海马神经元损伤较重;E组和F组线粒体体密度、数密度、比表面和嵴膜密度较A组明显减少(P<0.05),线粒体平均体积和平均截面积较A组明显增大(P<0.05)。结论:老龄大鼠脑缺血后产生延迟性神经元死亡,其线粒体的形态结构也发生明显变化,这些变化直接与脑缺血再灌注时程相关。
AIM:To observe ultrastructural change s in hippocampal neurons of the aged rats with incomplete cerebral ischemia /reperfusion and discuss charac ters and degrees of the morphological changes.METHODS:The aged SD rats were made as models fo r incomplete brain ischemia.The neuronal ultrastruct ural changes were observed by the tra ns-mission electron microscopy(TEM)at different time after reperfusion.The morphometric quantitative analysis on the mitochondria was analyzed by the computer image analysis system.RESULTS:Ultrastructures in hippocampal neu rons in group A was as same as group B.The neurons were damaged more severely in group E and F.The volume density,numerical density ,specific surface and cristae membra ne density of mitochondrial decreased obviously(P<0.05)and average vol-ume and sectional area increased rem arkably(P<0.05).
出处
《中国临床康复》
CSCD
2003年第28期3806-3807,T001,共3页
Chinese Journal of Clinical Rehabilitation
关键词
老龄大鼠
脑缺血再灌注
海马神经元
线粒体
动物模型
Ultrastructural changes of the mito chondria may be one of the important factors inducing dela yed neuronal death after ischemia /reperfusion.
