摘要
目的探讨临床脑脊液细胞学检查发现嗜中性粒细胞,对中枢神经系统感染的诊断价值。方法采用玻片离心沉淀法制片收集脑脊液细胞,瑞-姬姆萨染色(MGG),光学显微镜分类计数。结果159例脑脊液标本中细胞学检查有62例发现不同比例的嗜中性粒细胞(neutrophilic granulocytes,NG);脑脊液标本中发现NG可以见于多种神经系统疾病并以感染性疾病为主。其中化脓性脑膜炎(化脑)以均匀分布的NG为主,比例大多数为90%以上,平均为87.88±8.22%;结核性脑膜炎(结脑)以混合细胞反应为主,NG比例大多数在10%-80%,平均为35.57±22.95%;病毒性脑膜炎(病脑)很少出现NG,并且NG为0的敏感性为94.9%,特异性为57.7%。结论脑脊液细胞学检查中发现NG提示中枢神经系统感染的可能性较大;在临床应用中,对于鉴别病脑、化脑和结脑的意义较大。
Objective To observe the diagnostic value of neutrophilic granulocytes in cerebrospinal fluid in CNS infection. Methods Cells in cerebrospinal fluid were collected by centrifugation on the glass slide, then stained by Wright-Giemsa＇s, calculated the percentage of cell types by light microscope. Results In 62 of 159 cases, different ratio of neutrophilic granulocytes （NG） were tound in cerebrospinal fluid specimen. NGs could be found in many kinds of neurological diseases but mainly in infectious diseases. The ratios of uniform distributed NG mostly exceed 90%, and the average ratio is 87.88±8.22% in cerebrospinal fluid specimen of purulent meningitis;The ratios of NG in the mixing reactived cells mostly were in the interval of 10%-80% and the average ratio is 35.57± 22.95% in eerebrospinal fluid specimen of tuberculous meningitis. NG in majority cerebrospinal fluid specimen of viral meningitis were rarely found, if the ratios of NG were zero, the sensitivity was 94.9% and the specificity was 57.7% in diagnosis of viral meningitis. Conclusions NG found in eerebrospinal fluid specimen strongly suggested central nervous system infectious diseases, and could be used to distinguish purulent meningitis, tuberculous meningitis or viral meningitis.
出处
《安徽医学》
2009年第2期112-114,共3页
Anhui Medical Journal
基金
安徽医科大学第一附属医院院青年基金资助项目(2007)
关键词
脑膜炎
结核性
化脓性
病毒性
脑脊液细胞学
粒细胞
嗜中性
Meningitis,tuberculous,purulent,viral
Cerebrospinal fluid cytology
Granulocyte,neutrophilic
