摘要
[目的]探讨鹿茸多肽对β-淀粉样蛋白诱导脊髓神经元细胞凋亡的保护作用。[方法]体外进行胎鼠脊髓神经元细胞培养传代,细胞进入对数生长期后进行实验。MTT比色法检测不同浓度β-淀粉样蛋白作用24h后细胞活力变化;检测25μmol/Lβ-淀粉样蛋白作用24h后,细胞凋亡百分数和观察细胞caspase-3表达情况。[结果]不同浓度的β-淀粉样蛋白作用24h后对细胞活力均明显下降,并呈剂量依赖性(P<0.05);鹿茸多肽可明显抑制β-淀粉样蛋白诱导的脊髓神经元细胞凋亡现象(P<0.05),且可使caspase-3的表达量下降。[结论]鹿茸多肽通过抑制β-淀粉样蛋白诱导脊髓神经元细胞caspase-3表达增高而对其细胞凋亡发挥保护作用,本实验为探索新的治疗脊髓神经损伤的药物提供了重要的理论基础。
[Objective]To study effects of the velvet antler polypeptides on spinal nerve cell apoptosis induced by β-amyloid peptide. [Method]Spinal nerve cells were performed serial subcultivation, and entered into experiment during the exponential phase of growth. The viability of spinal nervecells 24h after induction by β-amyloid peptide with different concentrations were detected by MTT assay,percentages of the cell apoptosis and expression of casepase-3 were detected after induction by 25μmol/L β-amyloid peptide. [Result]It was revealed that 24 h after treatment with β-amyloid peptide of different concentrations, the viability of spinal nerve cells was significantly decreased in a dose-dependent manner（P〈0.05）. The velvet antler polypeptide may reduce apoptosis of spinal nerve cell induced by β-amyloid peptide. Then, the expression of casebase-3 was inhibited. [Conclusion]The velvet antler polypeptides may have protective effect on spinal nerve cell apoptosis induced by β-amyloid peptide via inhibiting the expression of caspase-3. Thus,it provides an important theoretic basis for the study of new drug of SCI.
出处
《浙江中医药大学学报》
CAS
2009年第1期45-47,共3页
Journal of Zhejiang Chinese Medical University
