摘要
目的探索用腺病毒作载体对脑胶质瘤进行基因治疗。方法构建含CMV启动子的HSV-tk基因的重组腺病毒载体,将HSV-tk基因转移至人脑胶质瘤细胞SHG_(44),用MTT方法测定GCV对肿瘤细胞的杀伤效果。结果转染HSV-tk基因的SHG_(44)细胞对GCV的敏感性增高80倍,MOT为100时,5mg/L的GCV能完全杀死肿瘤细胞,并存在旁观者效应,一个转基因细胞能引起10倍的亲本细胞对GCV敏感。结论腺病毒介导的HSV-tk/GCV系统能有效杀伤人脑胶质瘤细胞。
Objective To explore the treatment of human brain glioma using the adenovirus as a vector for gene transfer. Methods The recombinant adenovirus vector containing the HSV-tk gene controlled by the CMVpromoter was constructed. Then the vector was used to transfer the HSV-tk gene to the Chi- nese brain glioma cells-SHG44. The effectiveness of GCV to targeting cells was monitored by the method of MTT. Results The SHG44 cells transduced the HSV-tk gene by the adenovirus vectors were 80 times more sensitivety to GCV than the normal SHG44 cells. At the multiplicity of infection (MOD of 100, the cells would be killed at the concerntration of 5mg/L GCV. There also existed a potent by- stander effect that HSV-tk positive cells at a ratio of one positive to 10 HSV-tknegative SHG_(44)cells could be killed by coculture with HSV-tk negative cells. Conclusion The HSV-tk/GCV system mediated by adenovirus can kill human glioma cells efficiently.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
1998年第1期93-94,共2页
Chinese Journal of Experimental Surgery
基金
江苏省科委资助项目
