摘要
目的：探讨溶血磷脂酸（ＬＰＡ）刺激兔血管平滑肌细胞（ＶＳＭＣ）增殖的信号转导途径。方法：在培养的家兔血管平滑肌细胞上，测定３Ｈ胸腺嘧啶核苷（３ＨＴｄＲ）参入和丝裂素活化蛋白激酶（ＭＡＰＫ）活性。结果：ＬＰＡ呈浓度依赖地刺激３ＨＴｄＲ参入，并平行地激活ＭＡＰＫ，二者间呈显著正相关。乙二醇双（氨基乙酯）四乙酸（ＥＧＴＡ）、尼卡地平、ｒｙａｎｏｄｉｎｅ、ｔｈａｐｓｉｇａｒｇｉｎ、ｈｅｒｂｉｍｙｃｉｎＡ不影响ＬＰＡ促ＶＳＭＣ增殖作用。ＰＱ０９８０５９、Ｈ７、佛波酯（ＰＭＡ）及百日咳毒素（ＰＴＸ）均可抑制ＬＰＡ的促增殖和激活ＭＡＰＫ作用。结论：ＬＰＡ浓度依赖地促进ＶＳＭＣ增殖，其细胞内信号转导与ＰＴＸ敏感的Ｇ蛋白介导的蛋白激酶Ｃ（ＰＫＣ）和ＭＡＰＫ途径有关。
Objective:To investigate the signal transduction pathways stimulated by lysophosphatidic acid(LPA) in vascular smooth muscle cells(VSMC) of rabbits. Methods: The3HTdR incorporation and mitogenactivated protein kinase (MAPK) activity were measured in cultured VSMCs. Results: LPA enhanced the cultured VSMC3HTdR incorporation in a concentrationdependent manner, and increased MAPK activity concurrently. The relationship between3HTdR incorporation and MAPK activity induced by LPA showed significantly positive correlation. PQ098059,H7, PMA(preincubation) and pertussis toxin(PTX) inhibited the VSMC3HTdR incorporation and MAPK activity stimulated by LPA. However, EGTA, nicardipine, thapsigargin, ryanodine and herbimycin A had no effect. Conclusion: LPA has a stimulating effect on VSMCs proliferation; The LPAinduced intracellular signal transduction may be related to the pathway of G protein sensitive to PTX, which mediates protein kinase C(PKC) and MAPK activation.
出处
《北京医科大学学报》
CSCD
1998年第2期107-109,共3页
Journal of Peking University(Health Sciences)
基金
国家自然科学基金
关键词
溶血磷脂酸
血管平滑肌
细胞增殖
信号传递
Phosphatidic acids/pharmacol Muscle, smooth, vascular/drug eff Signal transduction Protein kinases/metab
