摘要
目的探讨纳米三硫化三砷（As2S2）与传统剂型As2S3对人骨髓增生异常综合征（MDS）细胞株MUTZ-1的生长抑制作用及其可能的机制。方法用MTT法比较两种剂型的As2S3对MUTZ-1细胞的生长抑制作用；透射电子显微镜技术分析细胞形态和超微结构的变化；流式细胞术分析细胞凋亡和周期的改变；发光比色法分析oaspase-3活性。结果2、4、8、16μmol／L两种剂型As2S3分别处理MUTZ-1细胞48h后，纳米As2S3对细胞的抑制率分别为48．9％、75．9％、89．4％和96．5％，而传统剂型As2S3组分别为14．5％、25．4％、34．7％和51．5％，曲组相比差异有统计学意义（P值均〈0．01）；纳米As2S3组细胞凋亡率分别为（12．9±1．9）％、（19．2±2．2）％、（30．1±2．5）％和（45．9±2．3）％，而传统剂型As2S3组分别为（5．3±1．8）％、（11．1±2．6）％、（19．3±2．3）％和（25．5±2．5）％，两组相比差异也有统计学意义（P值均〈0．01）；纳米As2S3组G2／M期细胞比例、caspase-3活性明显高于传统剂型As2S3组（P〈0．01），且以上各指标均呈浓度-时间依赖性。结论两种剂型As2S3对MUTZ-1细胞均有生长抑制和诱导凋亡作用，其机制可能与G2／M期细胞阻滞以及caspase-3活化有关；与传统剂型的As2S3相比，纳米As2S3有更强的抗肿瘤作用。
Objective To in vitro study the inhibition effect and possible mechanism of As2S3 nanop- articles（ As2S3 nano） on human MDS cell line MUTZ-1 and to compare with that of traditional As2S3. Method MUTZ-1 cells were treated with As2S3 nano and tranditional regular-sized particles （TRSP） at different concentrations. The cell growth inhibition rate was determined by MTT assay, cell apoptosis by morpbplogy and flow cytometry （FCM） , cell cycle by FCM and the activity of caspase-3 by chemiluminescence assay. Results Treatment of As2S3 nano and TRSP at concentrations of 2, 4, 8 and 16 μmoL/L for 48 h could lead to a significant dose-dependent decrease of MUTZ-1 cells and induce apoptosis. The percentages of inhibition were 48.9% , 75.9% , 89.4% and 96.5% in As2S3 nano vs 14.5% , 25.4% , 34.7% and 51.5% in TRSP and apoptosis rates were （12.9±1.9）%,（19.2±2.2）%,（30. 1±2.5）% and （45.9±2.3）% inAs2S3 nanovs （5.3±1.8%）%,（11.1 ±2.6）%, （19.3±2.3）% and （25.5±2.5）% in TRSP respectively. There was statistically significant difference in these two groups（ P 〈 0.01 ）. The proportion of cell in G2/M phase and the activity of easpase-3 of MUTZ-1 cells treated with As2S3 nano were significantly higher than those treated with control group and As2 S3 TRSP groups （ P 〈 0.01 ）. Conclusions As2 S3 nanoparticles and TRSP can inhibit the proliferation of MUTZ-1 cells and induce apoptosis, which maybe through activating caspase-3 pathways and increasing the proportion of G2/M phase. As2S3 nanoparticles can produce a much better antitter effect than As2S3 TRSP do.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
2009年第1期29-32,共4页
Chinese Journal of Hematology
基金
基金项目：国家“973”项目（2006CB705602）
国家自然科学基金（30671782）
