摘要
目的:探讨牙乳头细胞中存在的前体细胞类型及其分化特性。方法:分离培养出生后4 d的SD大鼠下颌第一磨牙牙乳头细胞,检测骨钙蛋白(osteocalc in,OCN)、牙本质涎蛋白(dentin sialoprote in,DSP)的表达。观察出生后1 d的SD仔鼠下颌第一磨牙牙胚、胚胎11 d的下颌突在肾被膜下生长2周后的情况。确定肾被膜下可作为移植区后,将牙乳头细胞团及与成釉器的复合体移植到肾被膜下,24 d后观察所形成的组织结构。结果:体外培养的牙乳头细胞可表达OCN和DSP。出生后1 d的SD仔鼠下颌第一磨牙牙胚、胚胎11 d的下颌突在肾被膜下可继续发育生长。经肾被膜下移植培养后,牙乳头细胞团可生成牙本质样结构和骨样结构,而与成釉器复合后只观察到紧邻支架的牙本质样结构。结论:牙乳头细胞中包含不同类型的前体细胞,其分化方向与所处微环境密切相关。
Objective：To study the types of progenitors in dental papilla cells （DPCs） and their differentiation characteristics. Methods： DPCs of the mandibular first molars of four-day post-natal SD rats were isolated and cultured. The expression of osteocalcin （OCN） and dentin sialoprotein （DSP） were detected using DAB kit. El 1 mandible primordias, tooth germs of the mandibular first molars of one-day post-natal SD rats, were isolated and transplanted under renal capsule respectively to observe whether they could survive and continue to develop. Then the pellets with and without dissociated enamel organ were trans- planted under renal capsule for 24 days. Results：Cultured DPCs could express positive OCN and DSP. After transplantation, the mandible primordias developed into cap stage. The continuous genesis of dentin and enamel increased the size of tooth germs. DPCs pellets formed dentin-like and bone-like structures, while pellets with enamel organ formed only dentin-like tissue adjacent to the frames. Conclusion ：There are different progenitors residing in DPCs. The differentiation was closely related to the surrounding environment.
出处
《北京大学学报（医学版）》
CAS
CSCD
北大核心
2009年第1期62-65,共4页
Journal of Peking University:Health Sciences
基金
国家自然科学基金资助(30801289)项目~~
关键词
牙乳头
细胞分化
肾
牙本质
Dental papilla
Cell differentiation
Kidney
Dentin
