摘要
目的:分析不同类型牙周炎患者唾液和集合龈下菌斑中3种厌氧微生物(包括牙龈卟啉单胞菌、福赛坦氏菌和齿垢密螺旋体)的检出率,并探讨唾液中3种微生物的存在状况与牙周临床指标的关系。方法:收集50例侵袭性牙周炎(aggressive periodontitis,AgP)、48例慢性牙周炎(chronic periodontitis,CP)患者和25例非牙周炎者的非刺激性唾液和集合龈下菌斑,应用PCR技术检测两种样本中的牙龈卟啉单胞菌、福赛坦氏菌和齿垢密螺旋体。结果:3种微生物在AgP组、CP组唾液和龈下菌斑中的检出率均显著高于非牙周炎组(P<0.01)。牙龈卟啉单胞菌在龈下菌斑和唾液中的检出率分别为:AgP组100%vs100%,CP组93.8%vs93.8%,非牙周炎组为32%vs48%。福赛坦氏菌在龈下菌斑和唾液中的检出率为:AgP组96%vs88%,CP组97.9%vs89.6%,非牙周炎组为32%vs24%。齿垢密螺旋体在龈下菌斑和唾液中的检出率为:AgP组94%vs86%,CP组89.6%vs70.8%,非牙周炎组12%vs16%。3种微生物在同一患者两种样本检测结果的一致性均较高。唾液中存在3种微生物均与牙龈出血指数密切相关,其中牙龈卟啉单胞菌的OR值高达13.5。结论:牙龈卟啉单胞菌、福赛坦氏菌和齿垢密螺旋体广泛存在于AgP和CP患者的唾液和龈下菌斑中。唾液中牙龈卟啉单胞菌、福赛坦氏菌和齿垢密螺旋体的检出状况与牙周临床指标密切相关,唾液样本可以用于口腔内牙龈卟啉单胞菌、福赛坦氏菌和齿垢密螺旋体的检测。
Objective：To investigate the prevalence of 3 anaerobic microorganisms including Porphyromonas gingivalis （ Pg ） , Tannerella forsythia （Tf） and Treponema denticola （Td） in saliva and pooled subgingival plaque of different types of periodontitis and compare the detections of three microorganisms between saliva and pooled subgingival plaque sample from the same patient, and analyze the relationship between prevalence of these pathogens in saliva and periodontal parameters. Methods：Non-stimulated saliva samples and pooled subgingival plaque samples were collected from 50 patients with aggressive periodontitis （AgP）, 48 patients with chronic periodontitis （CP） and 25 subjects with no periodontitis, and three microorganisms including Pg, Tf and Td were detected in these samples by PCR method. Results：Prevalence of three microorganisms in pooled subgingival plaque and saliva samples of AgP patients and CP patients were significantly higher than subjects with no periodontitis （ P 〈 0. 01 ）. The prevalence of Pg in Subgingival plaque and saliva was as follows ： AgP, 100% vs 100% ; CP, 93.8% vs 93.8% ; Subjects with no periodontitis, 32% vs 48% ）. The prevalence of Tf in Subgingival plaque and saliva was as follows： AgP, 96% vs 88% ; CP, 97.9% vs 89.6% ; Subjects with no periodontitis, 32% vs 24% ）. The prevalence of Td in Subgingival plaque and saliva was as follows： AgP, 94% vs 86% ; CP, 89.6% vs 70. 8% ; Subjects with no periodontitis, 12% vs 16% ）. The agreement between saliva and pooled subgingival plaque samples of these three microorganisms from the same patient was high. The presence of three microorganisms in saliva was all associated with bleeding index of gingiva, and odds ratio of Pg was 13.5 （P 〈0. 01 ）. Conclusion： Pg, Tfand Td were widely distributed in saliva and subgingival plaque of patients with AgP and CP. Presence of Pg, Tf and Td in saliva was related to periodontal parameters of gingiva, and saliva could be used as a promising sample for oral detection of these 3 microorganisms.
出处
《北京大学学报（医学版）》
CAS
CSCD
北大核心
2009年第1期44-48,共5页
Journal of Peking University:Health Sciences
基金
国家自然科学基金资助项目(30271411
30471882)
"十一五"国家科技支撑计划(2007BAZ18B02)
卫生部临床学科重点项目~~
关键词
牙周炎
唾液
牙菌斑
微生物学
Periodontitis
Saliva
Dental plaque
Microbiology
