摘要
目的：建立牙源性角化囊性瘤上皮细胞体外培养体系并进行初步鉴定。方法：采用组织块及酶消化法原代培养牙源性角化囊性瘤上皮细胞，以无血清的角化上皮细胞培养基进行体外连续培养，并行细胞形态学观察及免疫组织化学鉴定。结果：体外培养的牙源性角化囊性瘤上皮细胞为多角形，胞浆均匀，轮廓清晰，表现上皮细胞特有的铺路石样外观，体外可传2—4代，生长周期约30～50d。经波形丝蛋白、广谱角蛋白及角蛋白10、角蛋白14免疫组化染色证实，分离培养的细胞为上皮来源，无间充质细胞混杂。结论：采用无血清的角化细胞培养基可在体外进行牙源性角化囊性瘤上皮细胞的连续培养。
Objective：To establish a method of in vitro cultivation of epithelial cells of keratocystic odontogenic tumors （KCOT）. Methods： Tissue block and enzyme digestion techniques were used for primary cultivation of KCOT cells. The cells were grown in keratinocyte-serum free medium （K-SFM）. The biological characteristics of the cultivated keratinocytes were identified by phase microscopic observation and by immunohistochemistry of cytokeratin, vimentin, cytokeratin10 and 14. Results： KCOT keratinocytes could survive for 30 -50 days in K-SFM after passing 2 -4 generations. Cells were polygon and showed typical slabstone-like appearance. Immunostaining showed positive staining for cytokeratin antibody, and negative for vimentin. Conclusion： KCOT epithelial cells could be serially cultured in vitro in K-SFM by techniques suggested in this study.
出处
《北京大学学报（医学版）》
CAS
CSCD
北大核心
2009年第1期28-31,共4页
Journal of Peking University:Health Sciences
基金
国家自然科学基金(30625044和30572048)
高等学校博士学科点专项科研基金(20050001110)资助~~
关键词
牙源性囊肿
角化病
上皮细胞
细胞培养技术
Odontogenic cysts
keratosis
Epithelial cells
Cell culture techniques
