摘要
目的筛选及鉴定重症肌无力（ＭＧ）相关基因。方法应用差别显示ＰＣＲ（ＤＤＰＣＲ）技术比较１例ＭＧ患者受累骨骼肌和１例意外死亡者健康骨骼肌的基因表达，并用Ｎｏｒｔｈｅｒｎｂｌｏｔ研究此基因在６例ＭＧ患者和５名健康人骨骼肌和胸腺组织中的表达情况。结果经ＤＤＰＣＲ筛选到１个与ＭＧ相关的、全长约６ｋｂ的新基因，暂命名为ＬＰＷ１；Ｎｏｒｔｈｅｒｎｂｌｏｔ显示，在所有ＭＧ患者的骨骼肌组织和增生胸腺中，ＬＰＷ１ｍＲＮＡ表达水平均低于健康人组，而在胸腺瘤组织中的表达则高于健康人组；ＬＰＷ１不是骨骼肌特异的基因，在健康人肝、脑、胃、骨骼肌、胸腺和胎肾等组织中均有表达。结论ＬＰＷ１为ＭＧ相关基因，在ＭＧ的发病机制中可能起一定作用；伴发胸腺增生的ＭＧ和伴发胸腺瘤的ＭＧ的发生机制可能有所不同。
Objective To screen and identify the gene related to myasthenia gravis (MG). Methods Differential display PCR (DDPCR) was used to compare genes expression in muscles of one healthy control and one MG patient, and the mRNA levels of the related gene were analyzed in muscles and thymuses of MG patients. Results A new gene related to MG was found by using of DDPCR. Its full length was about 6 killobase. It was named LPW1 by us. Northern blot confirmed LPW1 mRNA levels were decreased in the skeletal muscle and hyperplasia thymuses but increased in thymoma of MG patients. It had not significantly difference between the type I, IIa and IIb of MG. Also, it did not correlate with anti AChR titer. The gene was not specific for the skeletal muscle, but also expressed in the liver, stomach, brain and fetal kidney. Conclusion The new gene, LPW1, might play an important role in the pathogenesis of MG, but there might be some difference between the pathogenesis of hyperplasia and thymoma in MG.
出处
《中华神经科杂志》
CAS
CSCD
1998年第2期69-71,共3页
Chinese Journal of Neurology
关键词
重症肌无力
聚合酶链反应
差别显示
基因
Myasthenia gravis Polymerase chain reaction, differential display Gene
