摘要
目的探讨热激蛋白70(HSP70)高表达在兔动脉粥样硬化斑块形成中的作用。方法30只6月龄健康兔,随机分为3组,均通过动脉内膜损伤术加高脂饮食形成动脉粥样硬化斑块。术后一组给以高脂饮食(对照组,C组),另一组给以高脂饮食加普伐他汀(普伐他汀1组,P1组),第三组术后2月内仅给以高脂饮食,第3个月起加普伐他汀(普伐他汀2组,P2组)。三组均通过超声检测斑块形成情况,测量腹主动脉内膜中层厚度(IMT)。饲养4个月后处死,取易损斑块,通过免疫组织化学技术检测斑块内HSP70高表达情况。结果兔腹主动脉IMT在三组均随时间延长逐渐变厚,但增厚程度不一,术后4个月后P1组、P2组明显小于C组(P<0.01);易损斑块主要见于C组和P2组;HSP70高表达阳性细胞在C组主要集中在纤维帽区与肩部区(P<0.01);普伐他汀干预后,HSP70高表达阳性细胞主要集中在肩部区(P<0.01),且纤维帽区HSP70高表达阳性细胞数明显减少(P<0.01)。结论HSP70在斑块肩部区、纤维帽区过度表达可导致炎症反应,降低斑块稳定性。
Objective To investigate HSP70 expression in vulnerable plaques in abdominal aorta the role of pravastation on its overexpression in model rabbits. Methods The atherosclerotic plaques were made in 30 rabbits by undergoing balloon-induced abdominal aorta wall injury and then being fed on a diet of high cholesterol. Then 30 rabbits were divided into three groups randomly ： one group were fed on a diet of high cholesterol （ group C）, and another were fed on Pravastatin besides cholesterol （ group P1 ）. The third were fed on a diet of high cholesterol in first two months,and Pravastatin was added to the diet in second two months. The intima-media thickness （IMT） and the atherosclerotic plaques were measured with ultrasound imaging before a diet of high cholesterol and half one month,one month,two months,three months and four months after abdominal aorta wall injuried. All rabbits were killed after 4 months,then vulnerable plaques were taken out, and overexpression of HSP70 was detected with SP immunohistochemical technique. Results IMT became thicker and thicker with time after the high cholesterol diet. The IMT of group P was signifidantly less than that of C group after d month post-injury （ P 〈 0.01 ）. Vulnerable plaques were main from both C and group P2. In group C, HSP70 overexpression cells were mainly focused on shoulder and fibrous cap regions （P 〈 0.01 ,P 〈 0.01 ） ;while in group P2 they were mainly focused on shoulder region （P 〈 0.01 ）, and the positive rate of HSP70 overexpression cells in fibrous cap in group P2 was significantly less than that of group C（ P 〈 0.01 ）. Conclusions There exists HSP70 overexpression in vnlnerable plaques of abdominal aorla, which can be decreased by pravastatin intervention.
出处
《中国临床保健杂志》
CAS
2009年第1期44-47,113,共5页
Chinese Journal of Clinical Healthcare
