摘要
目的:定量分析肝癌(HCC)组织及血中血管内皮生长因子(VEGF)表达,并探讨其临床意义。方法:按自身配对法收集人肝癌的癌灶、癌旁及远癌组织各36份;并收集肝癌、慢性肝炎、肝硬化患者和正常对照的外周血标本181份。以免疫组化法分析肝癌组织中VEGF表达、胞内分布和临床病理学特征,并以酶联免疫吸附法对肝癌组织和血VEGF浓度进行定量分析。结果:VEGF阳性表达呈棕黄色或棕褐色,分布于肝癌细胞胞浆中;肝癌组织、HCC无包膜者和HCC伴远处转移者VEGF阳性率分别为63.9%、78.3%和90.9%,不同直径HCC间及不同分化程度HCC间VEGF阳性率差异均无统计学意义(P>0.05);肝癌组织VEGF比浓度(ng/g肝)明显高于癌旁和远癌组织(P<0.01)。血VEGF如以>280ng/L为界,肝癌、慢性肝炎、肝硬化患者及正常对照组的阳性率分别为88.4%、14.3%、10.0%和0;血VEGF与AFP联检对肝癌的诊断阳性率为94.2%。结论:VEGF是反映肝癌侵袭生长及转移的有效指标。
Objective： To quantify the expression of vascular endothelial growth factor （VEGF） of liver and serum in hepatocellular carcinoma （HCC）, and to explore the clinical values. Methods： Thirty-six HCC, matched paracancerous and distal cancerous tissues were collected. One hundred and eighty-one peripheral venous blood were collected in patients with HCC, liver cirrhosis （LC）, chronic hepatitis （CH） and normal control （NC） respectively. Hepatic VEGF expression, cellular distributions and their clinical pathological features were investigated by immunohistochemistry. The VEGF levels of liver and serum were quantitatively detected by ELISA method. Results： The positive expression showed palm-yellow or palm- brown granules and distributed in hepatoeyte plasma of HCCs. The incidence of VEGF was 63.9% in HCC tissues, 78.3% in non-encapsulated HCCs, and 90.9% in HCCs with extrahepatic metastasis, respectively. No significant difference was found between hepatic VEGF and tumor size or differentiation degree （P 〉 0.05）. The specific concentration （ng/g liver） of HCC VEGF was significantly higher （P 〈 0.01） than that of paracaneerous or distal cancerous tissues. If the cut off values was more than 280 ng/L, the incidence of serum VEGF was 88.4% in HCC, 14.3% in CH, 10% in LC, and 0% in NC, respectively. Combined VEGF and AFP for HCC could increase up to 94.2%. Conclusion： VEGF is a useful marker for vascular invasion and metastasis of liver tumors.
出处
《天津医药》
CAS
北大核心
2009年第2期88-90,I0001,共4页
Tianjin Medical Journal
基金
江苏省卫生科技项目(项目编号:H200523)
南通市社会发展项目(项目编号:S2007031)
关键词
肝肿瘤
癌
血管内皮生长因子类
免疫组织化学
血清
liver neoplasms carcinoma vascular endothelial growth factors immunohistochemistry serum
