摘要
利用SSR标记技术对23份玉米自交系和4个测验种进行杂优类群研究。从117对引物中筛选出77对扩增带谱清晰且具有多态性的SSR引物,在供试材料中检测到398个等位基因变异,计算27个自交系间的遗传距离(GD)在0.1074～0.4380,平均0.2880。分析GD和产量发现,GD大于0.3100的组合具有明显产量优势,GD小于0.2880组合的产量优势较弱;根据分子聚群并结合产量和系谱来源分析,将27份自交系划分为3个杂种优势群A群、B群和C群。
Heterotic grouping of 23 maize inbred lines and 4 testers were classified by SSR markers. A set of 77 polymorphic SSR primers were selected from 117 primers, Genetic distances（GD） based on SSR markers of 27 lines ranging from 0.107 4 to 0.438 0, with an average of 0.288 0. Comparison of GD with yields, it could be found that the combinations with GD 〉 0.310 0 had great yield heterosis, while the combinations with GD 〈 0.288 0 had low yield heterosis. Combined with molecular markers and yield as well as pedigree data, the 27 inbred lines were classified into three heterotic groups A, B and C.
出处
《玉米科学》
CAS
CSCD
北大核心
2009年第1期54-58,共5页
Journal of Maize Sciences
基金
云南省自然科学基金项目(2004C0011Z)
