摘要
目的：探讨全反式视黄酸（ＡＴＲＡ）对乳腺癌细胞生长抑制和对细胞凋亡诱导的机理。方法：采用荧光显微术、流式细胞术、ＭＴＴ测定和ＲＮＡ印迹等方法，检测细胞凋亡、细胞生长和ｍＲＮＡ的表达水平。结果：１）ＡＴＲＡ能够有效地诱导ＺＲ－７５－１乳腺癌细胞凋亡，但不能诱导ＭＤＡ－ＭＢ－２３１乳腺癌细胞凋亡。２）ＡＴＲＡ能够有效地抑制ＺＲ－７５－１细胞的生长，但不能抑制ＭＤＡ－ＭＢ－２３１细胞生长。３）ＡＴＲＡ能够诱导ＺＲ－７５－１细胞的视黄酸受体ＲＡＲβ的表达，但不能诱导ＭＤＡ－ＭＢ－２３１细胞的ＲＡＲβ表达。结论：ＡＴＲＡ诱导的细胞凋亡与其对ＺＲ－７５－１细胞生长抑制有关，对ＭＤＡ－ＭＢ－２３１细胞生长及凋亡无影响，ＲＡＲβ的表达可能介导ＡＴＲＡ诱导的细胞凋亡和细胞生长抑制过程。
Objective: To explore the effect mechanism of all trans etinoic acid (ATRA) on induction of apoptosis and the growth inhibition in human breast cancer cell lines. Methods: Apoptosis, growth inhibition and mRNA level in breast cancer cell lines were detected by fluorescent microscopy, flow cytometry, MTT assay and northern blot. Results: 1) ATRA can induce apoptosis of ZR-75-1 breast cancer cells, but it can not induce apoptosis of MDA-MB-231 breast cancer cells. 2) ATRA can inhibit the growth of ZR-75-1 breast cancer cells effectively, whereas it has no effect on MDA-MB-231 breast cancer cells. 3) ATRA can induce RAR β expression in ZR-75-1 breast cancer cells, but it can not induce RAR β expression in MDA-MB-231 breast cancer cells. Conclusions: Induction of apoptosis may contribute to the growth inhibitory effect of ATRA in ZR-75-1 breast cancer cells. The expression of RAR β is an important factor which may mediate apoptosis induced by ATRA and growth inhibitory effect of ATRA.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
1998年第3期167-170,共4页
Chinese Journal of Cancer
基金
福建省自然科学基金
关键词
全反式视黄酸
细胞凋亡
乳腺癌
All-trans retinoic acid Apoptosis RAR β Breast cancer cell lines
