摘要
采用放射受体分析法确定，人肝癌细胞和正常肝细胞膜存在特异的亲合力不同的二类ＥＧＦ受体。进一步用化学交联法鉴定其受体分子量为１６８ｋＤａ。以３Ｈ－ＴｄＲ掺人和细胞计数为指标，无血清培养下，ＥＧＦ能明显地促进肝癌细胞的生长；ＥＧＦ抗体、ＥＧＦ受体单克隆抗体、生长抑素（ＳＳ）能抑制肝癌细胞的生长并拮抗ＥＧＦ的促生长作用。肝癌细胞在含ＳＳ的无血清培养基中培养后，其ＥＧＦ受体结合率降低。说明ＳＳ对ＥＧＦ受体有下调节作用。这可能是ＳＳ抑制肝癌细胞生长的机制之一。
GF receptors (EGFR) with two different affinities have been identified by radioreceptorassayin both human hepatoma cell line and plasma membranes of normal human liver. EGFR onhepatoma cells was further identified as a single protein band of l68,000 Daltons by means ofcovalent cross-linking and autoradiography. Using 3H-TdR uptake and cell counts as paramatersfor cell growth, EGF exerts a stimulatory effect on the growth of human hepatoma cells inserum-free medium. EGF-Ab, EGFR-McAb, and somatostatin(SS) all exert inhibitory effect on thegrowth of hepatoma cells, and SS has been shown to antagonize the growth-stimulatory effect ofEGF. After a 48-hr culture of hepatoma cells in serum-free medium containing SS, the binding of125I-EGF to EGFR on tumor cells was significantly reduced,indicating that SS has causeddown-regulation of EGFR. This is probably one of the mechanisms by which SS inhibits thegrowth of hepatoma cells.
出处
《中华消化杂志》
CSCD
北大核心
1995年第3期129-132,共4页
Chinese Journal of Digestion
关键词
肝肿瘤
表皮生长因子
生长抑素
人肝癌细胞株
Epidermal growth factor
Epidermal growth factor receptor
Somatostatin
.Human hepatoma cell line
Autocrine regulation.
