摘要
目的:观察中药复方含药血清对模拟失重条件下体外培养成骨细胞的影响,探讨该方对抗骨丢失的部分作用机理。方法:分离乳鼠颅骨成骨细胞,体外培养后,随机分为5%,10%,20%浓度的空白血清对照组和5%,10%,20%空白血清回转组和5%,10%,20%含药血清回转组,连续回转48h模拟失重,观察该方对大鼠成骨细胞增殖、I型胶原合成、碱性磷酸酶(ALP)和骨钙素(BGP)生成的影响。结果:5%和10%空白血清回转组成骨细胞增殖比正常重力组明显降低(P<0.05);与10%空白血清回转模拟失重组相比,10%含药血清回转组可显著促进细胞增殖(P<0.05)。正常重力组与回转模拟失重组Ⅰ型胶原含量比较差异无统计学意义(P>0.05);与5%和10%空白血清回转模拟失重组相比,5%和10%含药血清回转组均能明显促进成骨细胞Ⅰ型胶原合成(P<0.05)。与5%正常重力组相比,5%空白血清回转组成骨细胞ALP活性明显降低(P<0.05);与5%和20%空白血清回转模拟失重组相比,5%和20%含药血清回转组能明显提高成骨细胞ALP活性(P<0.05)。与10%、20%空白血清组相比,10%、20%空白血清回转组成骨细胞BGP含量明显降低(P<0.05);而中药复方含药血清对其模拟失重成骨细胞BGP活性没有显著影响(P>0.05)。结论:该方能改善模拟失重条件下成骨细胞增殖,分化成熟,增加胶原蛋白合成,从而实现对抗失重骨丢失的效果。
Objective：To study the effects of serum containing Chinese compound medicine on osteoblast under clinorotation --induced weightlessness （CIW）, so as to explore its mechanism of anti--bone loss. Mcthods：Osteoblasts were separated from cranial bones of neonate rats and cultured in vitro. The groupings were as follows： serum treated groups with the con- centrations of 5%, 10% and 20%, serum and CIW treated groups with 3 concentrations as above, and serum containing Chinese medicine compound plus CIW （48h） treated groups with the above 3 concentrations. The proliferation, synthesis of collagen type Ⅰ , alkaliphosphatase （ALP） and bone gla protein （BGP） of osteoblasts were observed. Results：Compared with cells in normal weight environment, osteoblast proliferation was obviously decreased in 5 % and 10 % serum groups （P〈%0.05）. Osteoblast proliferation was remarkably promoted in 10% serum containing medicine plus CIW group compared with 10% serum plus CIW group （P〈0.05）. There was no significant difference of type Ⅰ collagen between CIW group and normal group. Compared with 5% and 10 % serum and CIW groups, 5 % and 10% serum containing Chinese medicine compound plus CIW groups markedly improve the synthesis of type Ⅰ collagen in osteoblasts （P〈0.05）. Compared with 5% normal weight group, ALP activity in 5% serum and CIW group was statistically decreased （P〈0.05）. Compared with 5% and 20% serum and CIW groups, 5% and 20% serum containing Chinese medicine compound plus CIW groups remarkably increased ALP activity in osteoblasts （P〈0.05）. Compared with 10% and 20% serum groups, the BGP content of osteoblasts in 10% and 20% serum and CIW groups obviously decreased （P〈0.05）. However, serum containing Chinese medicine had no effect on BGP content in CIW osteoblasts （P〉0.05）. Conclusion： The formula can improve the proliferation, differentiation and collagen synthesis of osteoblasts in CIW environment, so as to inhabit bone loss in weight- lessness.
出处
《中国中医骨伤科杂志》
CAS
2009年第2期1-4,共4页
Chinese Journal of Traditional Medical Traumatology & Orthopedics
基金
国家自然科学基金(编号:30500663)
国家基础科学人才培养基金科研训练项目(编号:20070110)
北京市科技新星(计划A类)2006A49
教育部"新世纪优秀人才"NCET-06-0124
关键词
中药
失重模拟
成骨细胞
增殖
分化
Chinese medicine
Simulated weightlessness
Osteoblast
Proliferation
Differentiation
