摘要
目的了解医院临床分离的多药耐药铜绿假单胞菌中16S rRNA甲基化酶基因及氨基糖苷类修饰酶(AMEs)基因存在状况。方法收集山东和江苏两地医院临床分离的铜绿假单胞菌共50株(山东烟台多药耐药菌株30株,江苏泛耐药菌株20株),采用聚合酶链反应(PCR)及序列分析的方法分析6种16S rRNA甲基化酶(armA、rmtAr、mtB、rmtCr、mtD、npmA)和6种AMEs基因aac(3)-Ⅰ、aac(3)-Ⅱ、aac(6′)-Ⅰb、aac(6′)-Ⅱ、ant(3″)-Ⅰ和ant(2″)-Ⅰ。结果山东烟台30株多药耐药铜绿假单胞菌中4种基因aac(3)-Ⅱ、aac(6′)-Ⅱ、ant(3″)-Ⅰ和ant(2″)-Ⅰ的阳性株数分别为6株(20.0%)、14株(46.7%)、11株(36.7%)和1株(3.3%),其余8株基因均为阴性;AMEs基因总阳性率为50.0%;江苏20株泛耐药菌株中6种基因rmtB、aac(3)-Ⅱ、aac(6′)-Ⅰb、aac(6′)-Ⅱa、nt(3″)-Ⅰ和ant(2″)-Ⅰ的阳性株数分别为11株(55.0%)、12株(60.0%)、7株(35.0%)、12株(60.0%)、10株(50.0%)和9株(45.0%),其余6株基因均为阴性;AMEs基因总阳性率为95.0%。结论铜绿假单胞菌存在16S rRNA甲基化酶基因为国内首次报道;铜绿假单胞菌AMEs基因携带率很高;两所医院检出酶基因种类有差异。
OBJECTIVE To investigate the 16S rRNA methylases gene and AMEs of 30 strains multi-resistant Pseudomonas aeruginosa isolated from clinic work in our hospital. METHODS Collected 50 P. aeruginosa strains from two hospitals （30 strains of multi-resistant P. aeruginosa from Yantai, Shandong, 20 strains of pan-drug resistant P. aeruginosa from Jiangsu）, and analyzed 6 kinds of 16S rRNA methylases （armA,rmtA, rmtB, rmtC, rmtD and npmA） and 6 kinds of AMEs gene aac（3）-Ⅰ ,aac （3）-Ⅱ , aac（6′）-Ⅰ b, aac（6′）-Ⅱ , aac（3″）- Ⅰ , and aac （2″）-Ⅰ by PCR and sequence analysis. RESULTS Among 30 strains of multi-resistant P. aeruginosa from Yantai, the positive rate of 4 kinds of genes aac （3）-Ⅱ , aac（6′）-Ⅱ , aac （3″）-Ⅰ , and aac（2″） -Ⅰ was 20% （6/30）, 46.7% （14/30） ,36.7% （11/30） and 3.3%（1/30）. The other 8 kinds genes were all negative. The total positive rate of AMEs gene was 46.7% （14/30）. In 20 strains of pan-drug resistant P. aeruginosa, the positive rate of 6 kinds of genes rmtB, aac （3）-Ⅱ, aac（6′）-Ⅰb, aac（6r）-Ⅱ, aac（3″）-Ⅰ , and aac（2″）-Ⅰ was 55% （11/20）, 60% （12/20）, 35% （7/20）, 60% （12/20）,50% （10/20） and 45% （9/20）. The other 6 kinds genes were all negative. The total positive rate of AMEs gene was 95% （19/20）. CONCLUSIONS It is the first report that 16S rRNA methylases gene is existed in P. aeruginosa ; there is very high positive rate of AMEs genotypes in P. aeruginosa there are differences in gene existing among two hospitals.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2009年第2期138-141,共4页
Chinese Journal of Nosocomiology
基金
山东省技术发展攻关项目(2008GG10002040)
