摘要
目的观察重复取样后两次样本的碱性磷酸酶（ａｌｋａｌｉｎｅｐｈｏｓｐｈａｔａｓｅ，ＡＬＰ）水平的变化，比较袋口和袋内两种取样方法。方法分别用袋口和袋内法间隔２０ｍｉｎ重复取龈沟液（ｇｉｎｇｉｖａｌｃｒｅｖｉｃｕ－ｌａｒｆｌｕｉｄ，ＧＣＦ）样本，并测定其中的ＡＬＰ水平。结果两种方法取样示，２０ｍｉｎ后的ＡＬＰ水平与第１次相比差异无显著性（Ｐ＞０．０５）；袋内法获得的ＡＬＰ水平明显高于袋口法（Ｐ＜０．００１）；重复取样后，多数位点ＡＬＰ水平不能完全恢复，但袋内法略优于袋口法，用酶总量表达略优于用浓度表达。结论重复取样的间隔期宜超过２０ｍｉｎ；建议用袋内法取样测定ＡＬＰ水平并以酶总量表示为佳。
Objective To observe the change of alkaline phosphatase (ALP) after repeat sampling and compare the two sampling methods (orifice method and intra pocket method). Methods Gingival crevicular fluid (GCF) was sampled repeatedly by the interval of 20 minutes, with orifice and intra pocket method respectively, and the levels of ALP in GCF were determined. Results Mean levels of ALP per site and concentration of ALP are not statistically different between two samples after repeat sampling with orifice method as well as intra pocket method ( P >0.05). ALP levels (per site and per μl) are much higher for intra pocket method than orifice method ( P <0.001).From the viewpoint of the numbers of recovery sites after repeat sampling , though ALP levels at most sites can't entirly recover to their original levels with both method, intra pocket method is still a little better than orifice method, and ALP levels per site is better than ALP concentration. Conclusion The interval of repeat sampling should be prolonged more than 20 minutes. It is better to determine the GCF ALP with intra pocket method and also the recommended pattern that express enzyme levels is total activity per site but not concentration.
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
1998年第2期100-102,共3页
Chinese Journal of Stomatology
