摘要
在棘孢小单孢菌(Micromonospora echinospora)突变株(Fzu-707)产生小诺霉素的过程中,运用正交设计筛选小诺霉素(Micronomicin,MCR)生物合成促进剂——氨基酸.经摇瓶实验证明在原培养基中添加一定量的甘氨酸、赖氨酸、酪氨酸、蛋氨酸有利于生物合成小诺霉素,在旋转摇床上220 r/min 36℃培养,发酵时间由原工艺144 h缩短到104 h,产量(u.mL-1)较对照可增加20%左右,产素率(u.mL-1.h-1))提高60%左右.
The promoters （amino acids） were screened out by the orthogonal design in the course of biosynthesis micronomicin from the mutant strain （Fzu-707） of Micromonospora echinospora. Certain amount of glycine, lysine, tyrosine and methionine was added to the original medium in a flask on the rotary shaker 220 r/min and incubated at 36℃. It was propitious to biosynthesize micronomicin in cells of Micromonospora echinospora. The fermentation time was shortened from 144 h to 104 h, the yield of micronomicin （u · mL^-1） was increased about 20% compared with the control group and the production rate of micronomicin was increased about 60%.
关键词
小诺霉素
摇瓶发酵
产素率
氨基酸
micronomicin
fermentation in flask
production rate
amino acids
