摘要
目的观察库存血液保存天数与红细胞真性胆碱酯酶(E-ACHE)活力变化的关系,探讨其在有机磷中毒等抢救中的临床意义。方法选取无偿献血者23例,各采集全血200mL,置4℃冰箱按库存血液保存条件保存。逐日每份抽取全血1mL,测定E-ACHE,连续检测35d。结果库存血液随保存时间延长,E-ACHE水平有所波动,但无显著下降,各组间方差分析差异均无统计学意义(P>0.05);保存时间与E-ACHE水平直线回归方程差异也无统计学意义(P>0.05)。结论库存血E-ACHE活力水平,随保存时间延长,无显著下降,为临床应用提供了一定的实验依据,可供临床医生参考。
Objective To observe the relationship between the storage time of banked blood and the activity of erythrocyte true cholinesterase, so as to explore the clinical significance of erythrocyte true cholinesterase in rescuing of organophosphorus pesticides poisoning and so on. Methods Twenty-three blood donors without payment were enrolled in the investigation. Two hundred mL whole blood was respectively collected from each subject,and preserved in the 4 ℃refrigerator according to the banked blood storage standards. The value of erythrocyte true cholinesterase was detected with 1 mL whole blood extracting from each sample day by day for 35 consecutive days. Results With the prolongation of storage time of banked blood, the level of erythrocyte true cholinesterase fluctuated to a greater or less extent,but was not declined significantly,analysis of variance showed there was no statistical difference; so was the difference between the storage time and the linear regression equation （P〉0.05）. Conclusion With the prolongation of storage time,the level of erythrocyte true cholinesterase in the banked blood doesnr t decrease significantly, which provides some laboratory basis for its clinical application.
出处
《检验医学与临床》
CAS
2009年第2期90-91,共2页
Laboratory Medicine and Clinic
关键词
库存血
红细胞
真性胆碱酯酶
有机磷中毒
banked blood
erythrocyte
true cholinesterase
organophosphate poisoning
