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外周血胸苷酸合成酶mRNA和二氢嘧啶脱氢酶mRNA临床价值探讨 被引量：2

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摘要 目的探讨外周血胸苷酸合成酶(TS)mRNA和二氢嘧啶脱氢酶(DPD)mRNA检测的临床价值。方法用逆转录聚合酶链反应(RT-PCR)检测47例肿瘤患者和32例对照患者TS mRNA和DPD mRNA的表达,并作统计学分析。结果肿瘤组TS mRNA和DPD mRNA的表达率分别为27.7%和23.4%,对照组为9.38%和0.00%,肿瘤组高于对照组(P<0.05)。TS mRNA和DPD mRNA的表达水平与肿瘤的大小、有无淋巴结转移、组织学分级和临床分期均无相关性。结论用RT-PCR检测外周血TS mRNA和DPD mRNA,操作简便,可反复采样检测,便于临床追踪。
作者 陈贤冲 陶国华 仲崇俊 曹兴建 姜肖刚 谢玮
机构地区 南通市第一人民医院
出处 《检验医学》 CAS 北大核心 2009年第1期66-68,共3页 Laboratory Medicine
基金 江苏省社会发展指导性计划项目(BS2005626)
关键词 逆转录聚合酶链反应 胸苷酸合成酶 二氢嘧啶脱氢酶
分类号 Q503 [生物学—生物化学]
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参考文献11

  • 1Diasio RB. Improving fluorouracil chemotherapy with novel orally administered fluoropyrimidines[J]. Drugs, 1999,58 ( 13 ) : 119-126.
  • 2Diasio RB,Johnson MR. The role of pharmacogenetics and pharmacogenomics in cancer chemotherapy with 5-fluorouracil[J]. Pharmacology, 2000,61 ( 3 ) : 199- 203.
  • 3Lenz H J, Leichman CG, Danenberg KD, et al. Thymidylate synthase mRNA level in adenocarcinoma of the stomach: a predictor for primary tumor response and overall survival[J]. J Clin Oncol, 1996, 14 (1) : 176-182.
  • 4Liersch T, Langer C,Ghadimi BM. Lymph node status and TS gene expression are progno-stic markers in stage Ⅱ/Ⅲ rectal cancer after neoadjuvant fluorouracil-based chemoradiotherapy [J]. J Clin Oncol, 2006,24 (25) : 4062-4068.
  • 5Ricciardiello L,Ceccarelli C,Angiolini G,et al. High thymidylate synthase expression in colorectal cancer with microsatellite instability: implications for chemotherapeutic strategies [ J ]. Clin Cancer Res, 2005,11 ( 11 ) :4234-4240.
  • 6Ohrling K,Edler D, Hallstrom M,et al. Detection of thymidylate synthase expression in lymph node metastases of colorectal cancer can improve the prognostic information [J]. J Clin Oncol, 2005, 23 (24) : 5628-5634.
  • 7Lecomte T, Ferraz JM, Zinzindohoue F, et al. Thymidylate synthase gene polyinorphism predicts toxicity in colorectal cancer patients receiving 5-fluorouracil- based chemotherapy[J]. Clin Cancer Res, 2004,10 (17) :5880-5888.
  • 8Kommann M, Schwabe W, Sander S,et al. Thymidylate synthase and dihydropyrimidine dehydrogenase mRNA expression levels: predictors for survival in colorectal cancer patients receiving adjuvant 5-fluorouracil[ J ]. Clin Cancer Res, 2003,9 ( 11 ) : 4116- 4124.
  • 9Uetake H,Ichikawa W,Takeehi T, et al. Relationship between intratumoral dihydropyrimidine dehydrogenase activity and gene expression in human colorectal cancer[J]. Clin Cancer Res, 1999,5 ( 10 ) : 2836- 2839.
  • 10Nakagawa T,Tanaka F, Otake Y,et al. Prognostic value of thymidylate synthase expression in patients with p-stage Ⅰ adenocarcinoma of the lung [J]. Lung Cancer ,2002,35 ( 2 ) : 165-170.

同被引文献13

  • 1王国强,周志伟,万德森,潘志忠,李苏.血二氢嘧啶脱氢酶活性与结直肠癌临床病理参数关系的研究[J].大肠肛门病外科杂志,2004,10(3):180-182. 被引量：3
  • 2董秋美,何友兼,李苏,周中梅,张力,周志伟,夏忠军,李玉颜.结直肠癌患者DPD水平与5-FU血药浓度、疗效及毒性的相关性研究[J].癌症,2005,24(4):483-487. 被引量：9
  • 3彭柔君,董秋美,史艳侠,曹烨,周中梅,袁中玉,李苏,廖海,姜文奇.胃癌患者外周血DPD活性、5-FU血药浓度与毒性反应的相关性分析[J].癌症,2006,25(8):1039-1043. 被引量：8
  • 4DI PAOLO A,DANESI R,FALCONE A,et al.Relationship between 5-fluorouracil disposition,toxicity and dihydropyrimidine dehydrogenase activity in cancer patients[J].Ann Oncol,2001,12(9):1301-1306.
  • 5TERSHIMA M,FUJIWARA H,TAKAGANE A,et al.Prediction of sensitivity of fluoroprimidines by metabolic and target enzyme activities in gastric cancer[J].Gastric Cancer,2003,6(Suppl):71-81.
  • 6GAMELIN E,BOISDRON CELLE M,GURIN MEYER V,et al.Correlation between uracil and dihydrouracil plasma ratio,fluorouracil(5-FU) pharmacokinetic parameter,and tolerance in patients with advanced colorectal cancer:A potiential interest for prediciting 5-FU toxicity and determining optimal 5-FU dosage[J].J Clin Oncol,1999,17(4):1105.
  • 7KRISTENSEN M H,PEDERSEN P,MEJER J.The value of dihydrouracil/uracil plasma ratios in predicting 5-fluorouracil-related toxicity in colorectal cancer patients[J].J Int Med Res,2010,38(4):1313-1323.
  • 8BEN FREDJ R,GROSS E,BEN AHMED S,et al.The dihydrouracil/uracil ratio in plasma,clinical and genetic analysis for screening of dihydropyrimidine dehydrogenase deficiency in colorectal cancer patients treated with 5-fluorouracil[J].Pathol Biol,2009,57(6):470-476.
  • 9Lu ZH,Robert BD. Dihydropyrimidine Dehydrogenase Activity and Fluorouracil Chemotherapy[J].Journal of Clinical Oncology Edtorial,1994,(11):2239-2242.
  • 10Gamelin E,Boisdron-Celle M,Guerin-Meyer V. Correlation between Uracil and Dihydrouracil BloodRatio,Fluorouracil (5-FU)Pharmacokinetic Parameters,and Tolerance in Patients with Advanced colorectal Cancer:A Potential Interest for Predicting 5 FU Toxicity and Determining Optimal 5-FU Dosage[J].Journal of Clinical Oncology,1999,(04):1105-1110.

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检验医学
2009年 第1期

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