摘要
目的监测深圳口岸国际旅行人员中HIV感染者HIV-1亚型的流行状况。方法从69份HIV-1抗体阳性血浆中提取病毒总RNA,通过逆转录和巢式PCR技术扩增HIV-1gag和pol基因,再对PCR产物进行核苷酸序列测定,并对所获得的序列进行分析来确定基因亚型。结果69份样品中共发现9种HIV-1亚型和重组亚型,其中CRF01_AE重组亚型28份,B亚型21份,CRF02_AG重组亚型4份,C亚型4份,G亚型3份,CRF07_BC重组亚型3份,CRF08_BC重组亚型3份,A1亚型2份,D亚型1份。这些不同亚型的感染者来自包括中国在内的19个不同国家。结论深圳国际旅行人员中HIV-1亚型和重组亚型种类复杂多样,监测国际旅行人员中HIV感染者基因型分布情况,对及时发现新型毒株,预防和控制艾滋病十分重要。
Objective To monitor the epidemic status of HIV strains among HIV-infected international travelers at Shenzhen frontier port. Methods RNA was extracted from 69 HIV-1 positive plasmas, HIV-1 gag and pol genes were amplified by RT and nested-PCR. PCR product was sequenced directly. The acquired sequences were analyzed to determine the HIV-1 subtype. Results There were 9 kinds of HIV- 1 subtypes and recombinants in 69 samples, of which, 28 cases of CRF01_AE, 21 cases of B, 4 cases of CRF02_AG, 4 cases of C, 3 cases of G, 3 cases of CRF07_BC, 3 cases of CRF08_BC, 2 cases of A1, and 1 cases of D. These HIV infectors came from 19 countries including China. Conclusion The kind of HIV-1 subtype were complicated among Shenzhen international travelers. It is very important for HIV/AIDS prevention and control as well as surveillance of the genotypes of HIV strains among the international travelers and monitor new subtypes.
出处
《中国热带医学》
CAS
2009年第2期206-208,共3页
China Tropical Medicine
基金
深圳出入境检验检疫局科技计划项目(2007SZ2007017)
