摘要
选用了经初步筛选的 ２０个短随机序列引物（１０ ｎｔ）和 ２个长随机序列引物（２０ ｎｔ）对５个不同纬度来源的野生大豆（Ｇｌｙｃｉｎｅ ｓｏｊａ）进行了 ＤＮＡ随机扩增，其中有 １９个引物（含１个长引物）扩增出多态性片段，共获得７０个ＲＡＰＤ多态性片段，平均每个引物３．６８条．运用自编的计算机程序对扩增结果进行聚类分析（ＵＰＧＭＡ法）．结果表明，在野生大豆种群间存在一定的遗传变异，且种群间的遗传距离与地理纬度存在明显的相关性．这也为研究野生大豆种群间及进一步研究种群内的遗传多态性探索了一种简便的方法．
Random amplified polymorphic DNA (RAPD) analysis was applied to five populations of wild soybeans (Glycine soja ) originated from different latitudes. Altogether twenty screened random 10-mer primers and two random 20-mer primers were used to assay polymorphisms among populations. Nineteen primers including one 20-mer primer gave polymophic products and yielded seventy RAPD bands. Bands were scored (+/- ) and unweighted pair group method with arithmetic mean (UPGMA) was used to generate dendrogrmas showing genetic relationships among the G. soja populations evaluated. Cluster analysis showed there were some genetic variations among the G. soja populations and some noticeable correlations existed between the variations and the latitude.
出处
《复旦学报（自然科学版）》
CAS
CSCD
北大核心
1998年第2期208-212,共5页
Journal of Fudan University：Natural Science
基金
国家自然科学基金
