摘要
本文以沙田柚及其授粉树种酸柚为材料，对其花粉在不同培养介质中的萌发率进行测定，筛选了０２～０４ｍｏｌ／Ｌ聚乙二醇－４００（ＰＥＧ－４００）＋００１％硼酸作为花粉萌发介质。用授粉后３ｄ的花柱提取液进行沙田柚、酸柚花粉萌发抑制实验，花柱提取液对自交花粉萌发无抑制作用，明显地抑制花粉管的生长。其中以花柱提取液的３５％饱和度硫酸铵级分对花粉管伸长的抑制最明显。模拟了花粉在雌蕊中的生长方式，进行花粉萌发生长，能收集到较多的花粉管，为研究花粉管的Ｓ－糖蛋白提供了材料。
The pollen germination rates of shatinyu (Citrus grandis varshatinyu Hort.) and its pollinating plant common pomelo, in the various culture medium were measured0.2～0.4 mol/L PEG-400 and 0.01% H3BO3 was the culture medium for the pollen germinationThe inhibitive experiments of the pollen germination of the two materials were carried on within the stylar extracting solutions in the third day after pollinationThe solution didn′t inhibite the germination of the self-pollinated pollen, but it obviously inhibited the growth of the pollen tubeThe solution (at 35% ammonium bisulphate staging) very obviously inhibited the extension of the pollen tubeThe growth ways of the pollen in the pistil were initated, and the pollen germinated and grewThe pollen tubes were collected for the researches about S-glycoproteins in there.
出处
《广西植物》
CAS
CSCD
北大核心
1998年第2期160-164,共5页
Guihaia
基金
国家自然科学基金
广西自然科学基金
关键词
沙田柚
花柱蛋白
花粉管
生长抑制
自交不亲合性
Shatinyu (Citrus grandis varshatinyu Hort.)
stylar proteins
inhibition of the pollen tube
