摘要
为进一步探讨不同遗传背景的优良樱桃杂种优系再生体系的建立和通过基因工程选育樱桃抗性砧木,以F8、F10(Prunus.avium L.×Prunus.pseudocerasus L.)以及H8、H10(Prunus.cerasus L.×Prunus.pseudocerasus L.)等新品系为材料,MS和F14为基本培养基,通过添加不同浓度的6-苄基氨基腺嘌呤(6-BA)、3-吲哚丁酸(IBA)、赤霉素(GA3)进行杂种樱桃组织培养和快速繁殖技术的研究。各杂种优系在初代、继代、生根各阶段需要特定的培养基和激素配比。结果表明:F14+6-BA(0.3～0.5)mg/L+GA30.1(单位下同)适合各个杂种优系初代培养诱导出植株,成活率65%以上,F14+6-BA(0.5～1.0)+IBA(0.1～0.3)+GA30.1能够很好的促进各个杂种优系分化不定芽,增值系数在5.6倍以上,1/2MS+IBA0.5(或NAA0.8)是各个杂种优系的最佳生根培养基,生根率在83.3%以上。
In Vitro culture and micropropagation of different cherry hybrid selections including F8 and F10 （P. avium L.×P. pseudocerasus L.）, H8 and H10 （P. cerasus L.×P. pseudocerasus L.） were studied in this paper. The micropropagation program, including initial explant culture to create branched shoots, multiplication and rooting of in vitro shoots to produce plantlets, were successfully established by taking MS and F14 basic medium supplemented with different combinations of plant growth regulators such as 6-BA, IBA and GA3. It was showed that medium F14＋6-BA （0.3-0.5）mg/L＋GAa0.1mg/L was suitable for origin of the culture, with the survival rate of more than 65%. F14＋6-BA （0.5-1.0） mg/L ＋IBA （0.1--0.3） mg/L ＋GA30.1 mg/L was an optimal medium for proliferation of the In Vitro shoots, with the proliferating rate of 5.6 or more. Satisfactory rooting rate can be obtained on medium 1/2MS＋IBA0.5 （ or NAA 0.8） mg/L by more than 83.3%. This micropropagation program laid the foundation for further researches in establishing plant regeneration and genetic transformation system in cherry plants with different genetic background.
出处
《中国农学通报》
CSCD
北大核心
2009年第2期168-171,共4页
Chinese Agricultural Science Bulletin
基金
北京市自然科学基金"RNAi介导的抗李属坏死环斑病毒的樱桃转基因研究"(5072010)
北京市科技合同项目"转基因农作物新品种培育"(Z07070501770701)
北京市科技新星项目"与樱桃成花相关基因的克隆表达及遗传转化"(2007B042)
关键词
樱桃杂种
组织培养
快速繁殖
cherry hybrids, in vitro culture, micropropagation
