摘要
筛选确定了枯草芽孢杆菌BEM01产弹性蛋白酶培养基的碳源和氮源分别是葡萄糖和干酪素,吐温-80能刺激菌株生长和产酶。采用三因素三水平L9(33)正交试验优化了液体培养基葡萄糖、干酪素和吐温-80的浓度。结果表明,优化后的产酶培养基为:葡萄糖2%,干酪素2%,吐温0.01%,KH2PO40.2%,MgSO40.01%。优化后的发酵条件:发酵液初始pH为7.5,装样20 mL/300 mL三角瓶,按3%接种种子液,接种龄24 h,37℃180 r/min振荡培养36 h,菌株产弹性蛋白酶峰值为59 U/mL,较优化前酶活提高了31%。
The fermentation conditions were optimized in order to improve elastase production of Bacillus subtilis strain BEM01. The suitable carbon source and nitrogen source were sugar and casein, respectively, and tuween-80 was helpful to increase biomass and enzyme activity. Three- factor three-level orthogonal experiment was used to optimize the concentration of sugar, casein and tuween-80. The optimal liquid medium consisted of 2% sugar, 2% casein, 0.01% tuween-80, 0.2% KH2PO4 and 0.01% MgSO4. The optimum culture conditions were. initial pH 7.5, medium volume 20 mL in 300 mL flask, inoculation volume 3%, seed age 24 h and agitation speed 180 r/min. After incubation at 37℃ for 36 h, the elastase activity reached a maximum of 59 U/mL, which is 31% higher than before.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2008年第11期91-94,共4页
Food and Fermentation Industries
