摘要
目的:探讨西南地区雌激素受体α(estrogen receptorα,ERα)基因多态性与原性肝癌关系。方法:选择西南地区100名原发性肝癌患者为实验组,100名非肝病人群作为对照组。应用分子生物学的方法分析PvuⅡ,XbaⅠ限制性片段长度多态性(restriction fragment length polymorphism,RFLP)。同时对人雌激素受体基因上游的短串联重复序列(short tandem repeat,STR)进行纯化、克隆和序列分析,观察ERα基因多态性基因型在实验组与对照组中的基因型分布。结果:PvuⅡ和XbaⅠ限制性片段长度多态性在两组中均呈多态性分布。病例组TA13等位基因频率高于对照组,差异有显著性,TA15等位基因频率低于对照组,差异有显著性。结论:ERα基因多态性与原性肝癌有关,X等位基因可能是其危险因素,P等位基因可能是其保护因素,TA13等位基因可能是其危险因素,TA15等位基因可能是其保护因素。
Objective： To study the relationship between estrogen receptor α （ERα）gene polymorphism and primary liver cancer in South-Western China. Methods： We choose 100 no-hepatopathy person as control group and another 100 primary liver cancer as case group from South-Western China. Molecular biology is used to analyse restriction fragment length polymorphism （RFLP） of the first intron incision enzyme Pvu Ⅱ ,Xba Ⅰ in ERα gene. At the same time,we bring depuration,elone and sequence analysis to STR in hypervariable region of ERα gene. The ERα gene polymorphism gene- type distribution in ease group and control group was etudied. Results： RFLP of Pvu Ⅱ and Xba Ⅰ in the 2 groups are distributed with polymorphism. The TA13 allele frequency in case group is higher than that in control group,and the difference is significant. TA15 allele frequency in case group is lower than that in control group,and the difference is also significant（P= 0. 033）. Conclusion： ERα gene polymorphism has a relation with primary liver cancer. X allele may be the risk factor,and P allele may be its guard factor. TA13 allele may be the risk factor,and TA15 allele may be its guard factor.
出处
《华西医学》
CAS
2009年第1期70-73,共4页
West China Medical Journal
