摘要
目的研究刺五加多糖(Acanthopanacis senticosi polysaccharides,ASPS)对大鼠海马神经元抗氧化酶系统的影响。方法运用海马神经细胞原代培养技术,采用过氧化氢(H2O2)诱导建立细胞氧化损伤模型。实验共分为6组:阴性对照组、模型组和4个ASPS组(终浓度为10.00,5.00,2.50,1.25μg.mL-1)。观察细胞形态学变化;MTT法测定细胞活性;免疫组化法鉴定海马神经元,化学比色法测定细胞内超歧物过氧化物酶(SOD)、过氧化氢酶(CAT)及谷胱甘肽过氧化物酶(GSH-Px)的活力和丙二醛(MDA)含量。结果形态学观察显示:与模型组比较,ASPS组细胞损伤程度明显减轻;ASPS组细胞活性比模型组细胞活性高(P<0.01),SOD、CAT、GSH-Px活力ASPS组明显高于模型组,但MDA的含量ASPS组显著低于模型组。结论ASPS能提高海马神经元的抗氧化酶的活力,并对海马神经元氧化损伤有保护作用。
Objective To study the effects of Acanthopanacis senticosi polysaccharides on antioxidant enzyme system in hippocampal neurons of rats. Methods The model was induced by 1-1202 . The cell morphology was observed ; the viability of hippocampal neurons was detected with MTT method; and the hippocampal neuron was assessed by immunohistochemieal method. The activity of SOD, CAT, GSH-PX and content of MDA were determined by chemical-chromatometry. Results Compared with the model group, the morphology damage was slighter and the viability of hippocampal neurons was higher in ASPS groups （P 〈0.01 ）. The SOD,CAT,GSH-PX in ASPS groups were all more active than those in the model group, yet the content of MDA in ASPS groups was significantly lower than that in model group. Conclusion ASPS can increase the activity of antioxidase in hippocampal neurons, therefore protect the hippocampal neurons from oxidative damage.
出处
《医药导报》
CAS
2009年第1期21-24,共4页
Herald of Medicine
基金
湖北省科技攻关计划项目(基金编号:2006AA412C08)
关键词
刺五加多糖
海马神经细胞
抗氧化酶
Acanthopanacis senticosi polysaccharides
Hippocampal neurons
Antioxidase
