摘要
目的探讨阿司匹林(ASA)对脑缺血-再灌注(CIR)时心、肾损伤的保护作用及机制。方法线栓法制作大鼠局部脑缺血2h、再灌注24h模型,组织病理学观察心、肾损伤程度,生物化学方法测定三磷酸腺苷酶(ATPase)、乳酸脱氢酶(LDH)活性和乳酸(LA)含量,放射免疫法测定血浆前列环素I2(PGI2)和血栓素A2(TXA2)浓度。结果CIR时心肾均呈急性变质性改变,ASA抑制其形态学改变。CIR时,心肾的AT-Pase活性均明显升高,心肌LA含量和LDH下降,肾LDH升高,ASA提高了二器官Ca2+-ATPase活性,维持LDH于正常水平。结论阿司匹林对脑缺血-再灌注时心、肾损伤有明显保护作用,其机制可能与改善心肾组织代谢和维持器官灌流有关。
OBJECTIVE To investigate the protective effects and the mechanism of aspirin on heart and kidney after cerebral ischemia-reperfusion （CIR） for 24h in rats. METHODS Right middle cerebral artery was occluded by inserting a thread through internal carotid artery for 2h, and then reperfused for 24h. 60 mg· kg^-1 dose of aspirin was intragastric administrated at reperfusion Oh. The injured degree of heart and kidney was estimated by pathomorphologic method. The lactate content, lactate dehydrogenase（LDH）, and ATPase were measured by biochemical methods. The content of PGI2 and TXA2 in plasma was detected by^125I radio-immunoassay method. RESULTS After CIR 24h, the serious degeneration and necroses injured was shown in heart and kidney. ASA decreased the injury degree. After CIR, the activity of ATPase in heart and kidney was increased, the content of lactate and the activity of LDH in heart were decreased, the activity of LDH in kidney was increased. ASA increased the activity of Ca^2 ＋ -ATPase and balanced the activity of LDH in heart and kidney. CONCLUSION The protective effects of aspirin on heart and kidney with focal cerebral ischemia-reperfusion in rats might be attributed to its effects by improving the tissues metabolism and perfusion volume of heart and kidney.
出处
《海峡药学》
2008年第12期17-19,共3页
Strait Pharmaceutical Journal
关键词
阿司匹林
脑缺血-再灌注
心、肾损伤
乳酸
乳酸脱氢酶
ATPASE
Aspirin
Focal cerebral ischemia-reperfusion
Injury of heart and kidney
Lactate
Lactate dehydrogenase
ATPase
