摘要
目的探讨阿司匹林对脑缺血再灌注损伤炎症反应的影响及其机制。方法采用线栓法制备短暂性大脑中动脉缺血模型,用免疫组织化学染色观察脑组织中核转录因子-κB(NF-κB)活性、白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)的表达;HE染色观察中性粒细胞浸润。结果与对照组比较,小剂量阿司匹林组和大剂量阿司匹林组的NF-κB活性降低,以大剂量的阿司匹林作用更为明显;各阿司匹林组的IL-1β、TNF-α的表达明显降低,白细胞浸润明显减少。结论阿司匹林可抑制脑缺血再灌注损伤过程中的炎症反应;其机制可能与抑制NF-κB的激活和IL-1β、TNF-α表达有关。
Objective To study the effect of aspirin of inflammation in brain ischemic reperfution. Methods After MCA occlusion for 2 hours and reperfusion for 22 hours, the activity of NF-kB, the expression of TNF-α and IL-1β in brain tissue were measured by immunohistochemistry. The neutrophil in brain tissue was studied by HE stain. Results Compared with control group, aspirin, especially at high dose, inhibited the activity of NF-kB in brain. The expression of IL-1β and TNF-α in aspirin groups was lower signifieantly;The numbers of neutrophil in brain tissue in aspirin groups decreased significantly. Conclusions Aspirin, even at low dose,inhibits the acute inflammation in brain ischemic reperfusion injury, which is related to inhibition of the activity of NF-kB and the expression of TNF-α and IL-1β.
出处
《卒中与神经疾病》
2005年第5期262-264,268,共4页
Stroke and Nervous Diseases
