摘要
目的:研究重组蓝藻抗病毒蛋白N(CV-N)体外抗柯萨奇病毒B组3型(CVB3)的作用。方法:通过观察药物毒性、细胞病变效应(CPE)、MTT比色法检测细胞增殖,判断CV-N的细胞毒性及抗病毒效果。结果:CV-N对Vero细胞毒性较低(TC50为359μg/ml),对CVB3无直接灭活作用,对阻止CVB3病毒吸附细胞的作用优于病毒唑,而对病毒的复制无明显抑制作用。结论:CV-N作用于CVB3病毒感染的早期,有可能作为柯萨奇病毒感染的预防药物。
Objective： To investigate the antiviral activities of the recombinant cyanovirin-N （CV-N） against eoxsackie virus group B3 （ CVB3 ）. Methods ： The antiviral activities of CV-N in Vero cells were assessed by observation of cytotoxicity and cytopathie effect （CPE） and by MTY colorimetric assay of cell proliferation. Results： CV-N had slight cytotoxic effect on Vero cells （TCs0 = 359 Ixg/ml） and no direct inactivation on CVB3. It was more effective than Ribavirin in preventing CVB3 adsorption to Veto cells but showed no obvious inhibitory effect on CVB3 replication in host cells. Conclusion： CV-N exhibited a pronounced antiviral activity against CVB3 in the early stage of viral infection, and could be used to prevent CVB3infection.
出处
《医学研究生学报》
CAS
2008年第12期1242-1245,I0011,共5页
Journal of Medical Postgraduates
基金
山东省科技攻关资助项目(批准号:2005GG3202068)
