摘要
用与稻瘟病抗性基因Pi-d(t)紧密连锁的SSR标记引物RM262对云南地方核心稻种中的167个水稻品种(系)的抗稻瘟病基因Pi-d(t)进行检测。PCR扩增产物通过8%非变性聚丙烯酰胺凝胶电泳,结果显示扩增产物存在明显的多态,可分辨出4种带型;扩增产物在4%琼脂糖凝胶上只表现2种带型。选取17个品种(系)于温室接种致病菌系ZB13进行鉴定,确定PCR扩增片段大小(主带)约140和145 bp的水稻品种(系)不含Pi-d(t)基因,而扩增产物大小(主带)约150和155 bp的水稻品种(系)含有Pi-d(t)基因。实验明确了Pi-d(t)基因在云南地方核心稻种及其籼粳亚种中的分布情况,可为抗病育种和稻瘟病的防治提供理论依据。
The identification of the rice blast resistence gene Pi-d（t） in 167 accessions core collection of rice landraee in Yunnan was operated with the primer RM262 of SSR marker. The results showed that four types of DNA fragments were identified by 8% non-denaturing poly-acrylamide gel electrophoresis （PAGE） and only two types by 4% agarose gel electrophoresis. The results of pathogenicity assays of 17 rice cultivars with the rice blast fungus strains ZB13 in greenhouse showed that, the rice varieties in which PCR amplified fractions were 150 and 155 bp had rice blast resistance gene Pi-d（t） and the others in which PCR amplified fractions were 140 and 145 bp had not. In the study, the distribution of Pi-d （t） gene in the core germplasm of Yunnan indigenous rice varieties, Indica and Japonica was determined, which could provide theoretical evidence for resistance breeding disease and controlling rice blast.
出处
《西南农业学报》
CSCD
2008年第6期1583-1586,共4页
Southwest China Journal of Agricultural Sciences
基金
云南省“十一五”攻关计划项目资助
