摘要
应用水稻稻瘟病抗性基因 [Pi d(t) ]紧密连锁的微卫星标记RM 2 6 2对含有该抗病基因的品种地谷与感病品种江南香糯和 8987的杂交F2群体进行遗传分析和抗性鉴定 ,结果表明 ,RM2 6 2的PCR扩增物在抗、感品种之间的多态性较好 ;在 2个F2群体中 ,RM2 6 2和抗病基因间的重组率分别为 5 74%和 8 17% ,应用该标记的抗性纯合和杂合带型选择抗性植株 ,其准确率可达 98%以上。此外 。
The microsatellite (SSLP) marker RM262,which is tightly linked to the blast resistance gene Pi\|d(t) in rice,provides means to conduct marker\|aided selection in a rice breeding program. The objective of this study was to investigate the ability of this marker to select the blast resistance gene,Pi\|d(t),in two crosses for rice blast resistance breeding. The products with amplified the microsatellite primers were polymorphic between the three varietes examined. To examine the power of the identified microsatellite marker in predicting the Pi\|d(t) locus,we determined the genotypes of the two F\-2 populations at the Pi\|d(t) locus by performing progeny testing for the disease respones. The results indicated an accuracy of more than 98% in identifying the resistant plants in both populations. Therefore the microsatellite marker can be utilized in marker\|assisted selection and breeding for new varieties with blast resistance.
出处
《生物工程学报》
CAS
CSCD
北大核心
2000年第3期324-327,共4页
Chinese Journal of Biotechnology
基金
国家自然科学基金资助项目! ( 3 980 0 10 2 )
关键词
水稻
稻温病
辅助育种
微卫星标记
抗病基因
Rice,blast resistance gene,microsatellite marker,marker\|assisted selection
