摘要
目的探讨通脉口服液对实验性糖尿病肾病(DN)模型大鼠的肾脏保护作用。方法SD雄性大鼠予链脲佐菌素单次腹腔注射及隔日高脂饲料造模,筛选DN成模大鼠,随机分组,药物干预;6周后处死大鼠,检测尿蛋白、血糖、肾功能、血脂,电镜及光镜观测肾脏病理变化,测量肾小球截面积(MGA)、细胞外基质截面积(MMA)、肾小球基底膜(GBM)厚度,计算细胞外基质截面积/肾小球截面积(MMA/MGA)比值。结果通脉组大鼠24h尿蛋白定量、K/W比值、GBM、MMA/MGA比值较模型组均明显降低,光镜、电镜示病理损害较模型组改善;通脉组血脂较洛汀新组、模型组降低。结论益气活血中药复方通脉口服液可减轻实验性DN模型大鼠蛋白尿及肾脏损害,改善脂质代谢,发挥肾脏保护作用。
Objective： To observe the renal protection effect on rats of the diabetic nephropathy treated by Tongmai Oral Solution. Methods： SD male adult rats of specified -pathogen free were injected with streptozotoein （STZ） intraperitoneally to prepare for diabetes mellitus animal model and then randomly divided into 3 groups including model group, Tongmai Oral Solution group and lotensin group after diabetes was induced successfully. All rats were raised with high fat forage. All the rats were observed closely for 6 weeks. The urinary protein, GLU, blood - fat were recorded. The pathological changes of kidney were observed, and the MGA, MMA, GBM were recorded. Results： The quantity of urinary protein of 24h of Tongmai Oral Solution group was less than that of model group with statistical significance, as well as the ratio of K/W, GBM and MMA/MGA. Pathological changes of kidney in Tongmai Oral Solution group was less than that of model group. The TCh and TG of Toagmai Oral Solution group was less than that of model group and lotensin group with statistical significance. Conclusion： Tongmai Oral Solution can lessen the urinary protein and renal injury of DN rats, as well as improving the fat metabolism and taking the role as renal protection.
出处
《中国中医急症》
2009年第1期93-95,共3页
Journal of Emergency in Traditional Chinese Medicine
关键词
糖尿病
肾病
通脉口服液
大鼠
药理学
Diabetic
Nephropathy
Tongmai Oral Solution
Rat
Pharmacology
