摘要
目的:探讨柿叶水提物对H_(22)荷瘤小鼠和S_(180)荷瘤小鼠的抗肿瘤作用。方法:建立H_(22)荷瘤小鼠和S_(180)荷瘤小鼠移植瘤模型,观察分析柿叶水提物各组对荷瘤小鼠的生命延长率、抑瘤率、胸腺指数、脾脏指数、肿瘤坏死因子(TNF-α)、超氧化物歧化酶(SOD)、丙二醛(MDA)、一氧化氮(NO)的影响。结果:柿叶水提物高、中、低剂量组及环磷酰胺组对H_(22)荷瘤小鼠的生命延长率分别为34.05%,21.08%,5.95%,51.89%,对S_(180)荷瘤小鼠的生命延长率分别为30.11%,17.74%,4.30%,45.16%。柿叶水提物高、中、低剂量组及环磷酰胺组的抑瘤率分别为42.08%,25.04%,23.95%,72.77%。柿叶水提物高剂量组的胸腺指数为(3.9±1.0)mg·g^(-1),较氯化钠溶液组(3.0±1.1)mg·g^(-1)提高(P<0.05),柿叶水提物高剂量组的脾脏指数为(11.8±1.9)mg·g^(-1),较氯化钠溶液组(9.2±1.6)mg·g^(-1)高(P<0.05)。柿叶水提物高、中、低剂量组TNF-α数值分别为(1.94±0.20),(1.89±0.54), (1.32±0.18)ng·ml^(-1),较氯化钠溶液组(1.18±2.0)ng·ml^(-1)高(P<0.05)。柿叶水提物高、中、低剂量组SOD数值分别为(312.4±15.06),(304.1±16.1),(301.1±19.22)U·ml^(-1),较氯化钠溶液组(278.1±11.02)U·ml^(-1)高(P<0.01或P<0.05)。柿叶水提物高、中、低剂量组MDA数值分别为(5.85±1.12),(6.31±1.17),(6.62±1.03)nmol·ml^(-1),较氯化钠溶液组(7.26±0.62)nmol·ml^(-1)下降(P<0.01或P<0.05)。柿叶水提物高、中、低剂量组NO数值分别为(47.0±7.46),(51.2±9.07),(56.1±9.98)μmol·L^(-1),较氯化钠溶液组(62.2±1.22)μmol·L^(-1)下降(P<0.01或P<0.05)。结论:柿叶水提物有一定的抗肿瘤作用,其作用机制可能与调节细胞因子TNF-α的分泌、增强免疫功能和抗氧化有关。
ABSTRACT Objective： To study the antitumor effects of water extract of persimmon leaves in H22-bearing mice and S180-bearing mice. Method： H22-bearing mice and S180-bearing mice were established and divided into five groups： normal saline （NS） group, cyelophosphamide （CTX） group, high, middle and low dose of water extract of persimmon leaves groups. The life prolongation rate , tumor inhibition rates, values of thymus index, spleen index, Tumor necrosis factor-α（TNF-α）, superoxide dismutase（SOD）, maleic dialdehyde（MDA） and nitrogen monoxidum（NO） were recorded, observed and analyzed. Result： The life prolongation rate of H22- bearing mice in high, middle, low dose groups and CTX group was 34.05%, 21.08%, 5.95% and 51.89%, respectively,and, the life prolongation rate of Sls0-bearing mice in high, middle, low dose groups and CTX group was 30. 11% , 17.74% , 4.30% and 45.16% respectively. The tumor inhibition rates in high, middle, low dose groups and CTX group were, 42.08% , 25.04% , 23.95% and 72.77%, respectively. The thymus index was significantly higher in high dose groups than that of in NS group[（3.9±1.0 ） rag. g^-1 vs. （ 3.0 ± 1.1 ） rag. g^-1, P 〈 0.051, corresponsively , the spleen index of high dose group was higher than that of NS group [ （ 11.8 ± 1.9 ） mg· g^-1 vs. （ 9.2± 1.6） mg· g^-1, p 〈 0.05 ]. TNF-α of high, middle and low dose groups [ （ 1.94 ± 0.20） ng· ml^-1, （ 1.89 ± 0.54） ng·ml ^-1 and （ 1.32 ± 0.18 ） ng· ml^-1] were higher than that of NS group [ （ 1.18 -＋ 2.0） ng· ml -＇ ] （ P 〈 0.05 ）. SOD of high, middle and low dose groups [ （ 312.4 ±15.06） U·ml^-1, （ 304.1 -＋ 16.1 ） U·ml^-1, （ 301.1 ± 19.22 ） U·ml^-1 ] were higher than that of NS group [ （ 278.1 ± 11.02） U· ml^- 1 ] （ p 〈 0.01 or P 〈 0.05 ）. Corresponsively, MDA was lower in high, middle and low dose groups[ （5.85 ± 1.12）nmol·ml ^-1, （6.31 ± 1.17） nmol·m1^-1 and （6.62 - 1.03） nmol·ml^-1, respectively] than that in NS group [（7.26 ± 0.62） nmol·ml^-1]（P 〈 0.01 or P 〈 0.05 ）. NO was lower in high, middle and low dose groups [（47.0 ± 7.46） μmol · L^-1, （ 51.2 ＋ 9.07 ） μmol·L^-1, （56.1 ＋ 9.98 ） μmol· L^-1, respectively ] than that in NS group [（ 62.2 ± 1.22 ） μmol·L^-1] （ P 〈 0.01 or P 〈 0.05 ） . Conclusion ： Water extract of persimmon leaves possess certain antitumor effects, and its mechanism may be related to the effects of adjusting secretion of TNF-α, promotion immune responses and increasing antioxidation.
出处
《中国药师》
CAS
2009年第1期31-33,共3页
China Pharmacist
