摘要
目的:比较酶联免疫吸附试验(ELISA)法和微粒子酶免疫分析(MEIA)法测定血清乙型肝炎病毒e抗原(HBeAg)的检测性能。方法:用ELISA和MEIA法分别检测临床上已确诊的386份乙肝患者血清中的HBeAg。结果:ELISA法检出的HBeAg阳性率为49.2%,与ELISA法比较,MEIA法可显著提高HBeAg的阳性检出率(65%,χ2=161.09,P<0.01)。与ELISA法比较,MEIA法可检出更高稀释浓度血清中的HBeAg。结论:与ELISA法比较,MEIA法可大大提高测定血清HBeAg的灵敏度和准确性。
Objective： To compare the performance of enzyme-linked immunosorbent assay （ELISA） and microparticle enzyme immunoassay （MEIA） in detecting serum HBeAg. Methods： 386 serum samples from the patients diagnosed as viral hepatitis type B were tested by MEIA and ELISA respectively. Results： The positive rate of HBeAg by ELISA was 49.2%, and that by MEIA was 65%, a significant difference was shown between them （Х^2=161.09,P〈0.01）. Compared with ELISA, MEIA could detect the serum HBeAg in samples with higher diluted times. Conclusion： MEIA exhibits higher sensitivity and accuracy in detecting the serum HBeAg than ELISA.
出处
《中国医药导报》
CAS
2009年第1期75-75,79,共2页
China Medical Herald
